{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:25:50Z","timestamp":1770909950748,"version":"3.50.1"},"posted":{"date-parts":[[2025,1,2]]},"group-title":"Rheumatology","reference-count":24,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T00:00:00Z","timestamp":1735776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,1,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Granulomatous myositis (GM) is defined by focal collections of activated macrophages that fuse to form multinucleated cells that aggregate into granulomas within skeletal muscle. This study aimed to elucidate the pathophysiology of GM by defining its specific transcriptomic profile.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Bulk RNA sequencing was performed on 722 muscle biopsies, including 38 from patients with GM, other myopathies, and healthy comparators. Spatial transcriptomics and immunohistochemistry assays were used to identify the location of specific transcripts and proteins within the tissue.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The transcriptomic signature of GM muscle biopsies was characterized by high levels of IFN\u03b3, IFN\u03b3-inducible genes, and proinflammatory cytokine genes including IL1B, TNF, and TGFB1. The expression levels of 1293 specifically upregulated and 256 specifically downregulated genes were highly correlated with transcriptomic markers of disease activity. Support vector machine models using this gene set identified GM with an AUC of 99.6% (99.0%-99.9%) and an accuracy of 98.6% (98.2%-98.9%). Expression of CHIT1, the most significantly upregulated gene, strongly correlated with disease severity and was detected at the RNA and protein level in granulomas and giant cells.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>GM is transcriptomically characterized by a strong IFN\u03b3 signature and overexpression of proinflammatory cytokines, including IL1B, TNF, and TGFB1. Additionally, it exhibits a unique transcriptomic profile, including CHIT1, which correlates with disease activity.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.12.27.24319708","type":"posted-content","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T07:12:30Z","timestamp":1736147550000},"source":"Crossref","is-referenced-by-count":1,"title":["Identification of a distinctive gene signature in granulomatous myositis"],"prefix":"10.64898","author":[{"given":"Iago","family":"Pinal-Fernandez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolas","family":"Ruffer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Casal-Dominguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Pak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Kleefeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinna","family":"Preusse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael","family":"Kirou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Travis B.","family":"Kinder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania Del","family":"Orso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faiza","family":"Naz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamima","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Gutierrez-Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita","family":"Milone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Selva-O\u2019Callaghan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose C.","family":"Milisenda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teerin","family":"Liewluck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Stenzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew L.","family":"Mammen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025010700500456000_2024.12.27.24319708v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30254-0"},{"key":"2025010700500456000_2024.12.27.24319708v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2024.06.007"},{"key":"2025010700500456000_2024.12.27.24319708v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keac668"},{"key":"2025010700500456000_2024.12.27.24319708v1.4","doi-asserted-by":"publisher","DOI":"10.1136\/ard-2024-225773"},{"key":"2025010700500456000_2024.12.27.24319708v1.5","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2019-216599"},{"key":"2025010700500456000_2024.12.27.24319708v1.6","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.0000000000008128"},{"key":"2025010700500456000_2024.12.27.24319708v1.7","doi-asserted-by":"publisher","DOI":"10.1136\/ard-2023-223873"},{"key":"2025010700500456000_2024.12.27.24319708v1.8","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20464"},{"key":"2025010700500456000_2024.12.27.24319708v1.9","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000000642"},{"key":"2025010700500456000_2024.12.27.24319708v1.10","doi-asserted-by":"publisher","DOI":"10.1002\/art.41964"},{"key":"2025010700500456000_2024.12.27.24319708v1.11","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211468"},{"key":"2025010700500456000_2024.12.27.24319708v1.12","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-021-02365-5"},{"key":"2025010700500456000_2024.12.27.24319708v1.13","unstructured":"Dubowitz V , Sewry C , Oldfors A . 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