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To characterize the mechanisms underlying tumor-host interactions, we utilized a BioID-based proximity labeling method to identify proteins secreted by Yki\n                  <jats:sup>act<\/jats:sup>\n                  adult\n                  <jats:italic>Drosophila<\/jats:italic>\n                  gut tumors into the bloodstream\/hemolymph. Among the major proteins identified are coagulation and immune-responsive factors that contribute to the systemic wasting phenotypes associated with Yki\n                  <jats:sup>act<\/jats:sup>\n                  tumors. The effect of innate immunity factors is mediated by NF\u03baB transcription factors Relish, dorsal, and Dif, which in turn upregulate the expression of the cachectic factors Pvf1, Impl2, and Upd3. In addition, Yki\n                  <jats:sup>act<\/jats:sup>\n                  tumors secrete Eiger, a TNF-alpha homolog, which activates the JNK signaling pathway in neighboring non-tumor cells, leading to cell death. The release of damage-associated molecular patterns (DAMPs) from these dying cells presumably amplifies the inflammatory response, exacerbating systemic wasting. Targeting the inflammatory response, the JNK pathway, or the production of cachectic factors could potentially alleviate the debilitating effects of cancer cachexia.\n                <\/jats:p>","DOI":"10.1101\/2025.01.07.631515","type":"posted-content","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T06:20:14Z","timestamp":1736317214000},"source":"Crossref","is-referenced-by-count":5,"title":["Cell-death induced immune response and coagulopathy promote cachexia in\n                  <i>Drosophila<\/i>"],"prefix":"10.64898","author":[{"given":"Ankita","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, 7 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1494-1402","authenticated-orcid":false,"given":"Yanhui","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Genetics, Blavatnik Institute, Harvard Medical 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