{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T06:47:20Z","timestamp":1776062840556,"version":"3.50.1"},"posted":{"date-parts":[[2025,1,17]]},"group-title":"Bioengineering","reference-count":80,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The gut microbiota influences systemic immunity and the function of distal tissues, including the brain, liver, skin, lung, and muscle. However, the role of the gut microbiota in the foreign body response (FBR) and fibrosis around medical implants is largely unexplored. To investigate this connection, we perturbed the homeostasis of the murine gut microbiota via enterotoxigenic\n                  <jats:italic>Bacteroides fragilis<\/jats:italic>\n                  (ETBF) infection and implanted the synthetic polymer polycaprolactone (PCL) into a distal muscle injury. ETBF infection in mice led to increased neutrophil and \u03b3\u03b4 T cell infiltration into the PCL implant site. ETBF infection alone promoted systemic inflammation and increased levels of neutrophils in the blood, spleen, and bone marrow. At the PCL implant site, we found significant changes in the transcriptome of sorted fibroblasts but did not observe gross ETBF- induced differences in the fibrosis levels after 6 weeks. These results demonstrate the ability of the gut microbiota to mediate long-distance effects such as immune and stromal responses to a distal biomaterial implant.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Significance Statement<\/jats:title>\n                  <jats:p>The foreign body response to implants leads to chronic inflammation and fibrosis that can be highly variable in the general patient population. Here, we demonstrate that gut dysbiosis via enteric infection promoted systemic inflammation and increased immune cell recruitment to an anatomically distant implant site. These results implicate the gut microbiota as a potential source of variability in the clinical biomaterial response and illustrate that the local tissue environment can be influenced by host factors that modulate systemic interactions.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.01.13.632473","type":"posted-content","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T09:55:18Z","timestamp":1737107718000},"source":"Crossref","is-referenced-by-count":1,"title":["Murine gut microbiota dysbiosis via enteric infection modulates the foreign body response to a distal biomaterial implant"],"prefix":"10.64898","author":[{"given":"Brenda","family":"Yang","sequence":"first","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Rutkowski","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Ruta","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elise","family":"Gray-Gaillard","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"David R.","family":"Maestas","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean H.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavita","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinqun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoguang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allen","family":"Chen","sequence":"additional","affiliation":[{"name":"Zanvyl Krieger Mind\/Brain Institute, Johns Hopkins University, Baltimore, MD 21218"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connor D.","family":"Amelung","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Duke University, Durham, NC 27708"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joscelyn C.","family":"Mej\u00edas","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua S. 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