{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T03:52:06Z","timestamp":1782359526204,"version":"3.54.5"},"posted":{"date-parts":[[2025,1,16]]},"group-title":"Microbiology","reference-count":72,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yang Biomedical Scholar award"},{"DOI":"10.13039\/100000060","name":"NIAID","doi-asserted-by":"crossref","award":["K24AI134990"],"award-info":[{"award-number":["K24AI134990"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000865","name":"Bill & Melinda Gates Foundation","doi-asserted-by":"crossref","award":["INV-050353"],"award-info":[{"award-number":["INV-050353"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"name":"funds from the UNC Office for Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Aedes<\/jats:italic>\n                  mosquitoes are widely distributed across the Democratic Republic of Congo (DRC), and are major vectors of dengue (DENV), Zika, chikungunya (CHIKV), and yellow fever (YFV) viruses. While the high burden of malaria in the DRC receives considerable attention, arboviruses remain understudied. In the setting of recent CHIKV and YFV outbreaks in southwestern DRC, we collected\n                  <jats:italic>Aedes<\/jats:italic>\n                  mosquitoes in three areas of Kimpese, DRC, near the Angola border, to investigate their virome. Metagenomic and targeted sequencing of eight randomly selected field mosquito pools, comprising 155 mosquitoes from three collection sites, confirmed high-confidence DENV reads and human blood meals in six (75%) and eight (100%) pools, respectively. We find diverse mosquito viromes including other known and putative human and animal viruses. Our findings provide strong evidence of endemic DENV transmission along the DRC-Angola border and illustrate the potential of wild-caught mosquitoes for xenosurveillance of emerging pathogens.\n                <\/jats:p>","DOI":"10.1101\/2025.01.16.633031","type":"posted-content","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T02:50:32Z","timestamp":1737082232000},"source":"Crossref","is-referenced-by-count":2,"title":["Evidence of dengue virus transmission and a diverse\n                  <i>Aedes<\/i>\n                  mosquito virome on the Democratic Republic of Congo-Angola border"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0579-6955","authenticated-orcid":false,"given":"Wenqiao","family":"He","sequence":"first","affiliation":[{"name":"Institute for Global Health and Infectious Diseases and Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4885-2750","authenticated-orcid":false,"given":"Thierry","family":"Bobanga","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5942-1534","authenticated-orcid":false,"given":"Anne","family":"Piantadosi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA"},{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8308-5294","authenticated-orcid":false,"given":"Zachary R.","family":"Popkin-Hall","sequence":"additional","affiliation":[{"name":"Institute for Global Health and Infectious Diseases and Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4295-223X","authenticated-orcid":false,"given":"Fabien","family":"Vulu","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7974-1933","authenticated-orcid":false,"given":"Matthew H.","family":"Collins","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3871-1751","authenticated-orcid":false,"given":"Melchior M.","family":"Kashamuka","sequence":"additional","affiliation":[{"name":"Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7657-6901","authenticated-orcid":false,"given":"Antoinette K.","family":"Tshefu","sequence":"additional","affiliation":[{"name":"Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0591-0850","authenticated-orcid":false,"given":"Jonathan J.","family":"Juliano","sequence":"additional","affiliation":[{"name":"Institute for Global Health and Infectious Diseases and Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4465-9830","authenticated-orcid":false,"given":"Jonathan B.","family":"Parr","sequence":"additional","affiliation":[{"name":"Institute for Global Health and Infectious Diseases and Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026020906401135000_2025.01.16.633031v3.1","unstructured":"Vector-borne diseases. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/vector-borne-diseases."},{"key":"2026020906401135000_2025.01.16.633031v3.2","doi-asserted-by":"crossref","unstructured":"Kading, R. 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