{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T05:51:40Z","timestamp":1775886700323,"version":"3.50.1"},"posted":{"date-parts":[[2025,1,22]]},"group-title":"Cancer Biology","reference-count":94,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,1,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Sphingomyelin Synthase 1 (gene name:\n                  <jats:italic>SGMS1<\/jats:italic>\n                  ) participates in regulation of sphingolipid levels by synthesizing sphingomyelin from ceramide and phosphatidylcholine. Evidence have supported SGMS1\u2019s functions in regulating proliferation, cell cycle, cell death and migration. While its functions have begun to be explored, very little is known about upstream regulators. Here, we demonstrate that\n                  <jats:italic>SGMS1<\/jats:italic>\n                  is a direct gene target of the GATA1-TAL1 transcriptional complex in K562 erythroleukemic cells. A predicted GATA1 consensus DNA binding sequence was identified with in a newly characterized alternative\n                  <jats:italic>SGMS1<\/jats:italic>\n                  promoter (TSS-7 promoter) and ChIP analysis confirmed GATA1 occupancy on the promoter. Down-regulation of GATA1 in K562 cells significantly decreased SGMS1 expression and enzymatic activity, and ChIP-Seq analysis from ENCODE showed colocalized peaks for GATA1 and TAL1\n                  <jats:italic>(<\/jats:italic>\n                  a well-established GATA1 DNA binding partner) on the\n                  <jats:italic>SGMS1<\/jats:italic>\n                  gene. Analysis of publicly available datasets shows that elevated\n                  <jats:italic>GATA1, TAL1<\/jats:italic>\n                  and\n                  <jats:italic>SGMS1<\/jats:italic>\n                  expression not only clusters GATA1 positive chronic myelogenous leukemia cells (like K562), but also selectively identifies acute erythrocytic and megakaryocytic leukemias (M6 and M7 AML, respectively). Microarray gene expression analysis after down-regulation of\n                  <jats:italic>SGMS1<\/jats:italic>\n                  in M6 AML Hel cells revealed alteration of genes regulating G2\/M check point and mitotic spindle formation. This phenotype was functionally confirmed by the significant delay in G2\/M cell cycle progression of cells with\n                  <jats:italic>SGMS1<\/jats:italic>\n                  downregulation and sensitization to the clinically relevant anti-mitotic agent, Taxol. Altogether, these results identify\n                  <jats:italic>SGMS1<\/jats:italic>\n                  as a novel target of GATA1\/TAL1 transcriptional complex and they support a role for the GATA1\/TAL1\/SGMS1 axis in regulating transit through G2\/M. Importantly, results also point to combination of anti-mitotic agents and inhibition of SGMS1 as a potential novel therapeutic approach against the aggressive and resilient M6 AMLs.\n                <\/jats:p>\n                <jats:sec id=\"s1\">\n                  <jats:title>KEY POINTS<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>\n                        The Sphingomyelin Synthase 1 gene (\n                        <jats:italic>SGMS1<\/jats:italic>\n                        ) is a novel direct target of GATA1 and TAL1.\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        High\n                        <jats:italic>SGMS1<\/jats:italic>\n                        levels are associated with high\n                        <jats:italic>GATA1\/TAL1<\/jats:italic>\n                        expression and regulate cell cycle progression through the G2\/M checkpoint in GATA1\n                        <jats:sup>+<\/jats:sup>\n                        erythroleukemic Acute Myeloid Leukemia Hel cells.\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        High\n                        <jats:italic>SGMS1<\/jats:italic>\n                        is associated with lower probability of survival of patients with Acute Myeloid Leukemia and down-regulation of SGMS1 co-operates with microtubule targeting agents to induce cytotoxicity in GATA1 positive AML Hel cells.\n                      <\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.1101\/2025.01.19.633812","type":"posted-content","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T17:45:13Z","timestamp":1737567913000},"source":"Crossref","is-referenced-by-count":1,"title":["Regulation of cell cycle by the novel GATA1\/TAL1\/Sphingomyelin Synthase 1 (\n                  <i>SGMS1<\/i>\n                  ) transcriptional axis. Implications for anti-leukemic strategies"],"prefix":"10.64898","author":[{"given":"Yasharah","family":"Raza","sequence":"first","affiliation":[{"name":"Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sitapriya","family":"Moorthi","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gui-Qin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Chiappone","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, 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