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APP and its metabolites are secreted by cells in extracellular vesicles (EVs). To study the function of APP-containing EVs, we isolated App-EVs from rat primary neuronal conditioned media and proteomic analysis identified the Valosin-containing protein (Vcp) as molecular cargo. Pharmacological modulation of Vcp activity was found to alter App processing and global EV secretion in rat primary neurons. AD-associated knock-in\n                  <jats:italic>App<\/jats:italic>\n                  mutations were found to alter the abundance of App-EVs and the trafficking of App metabolites within App-EVs, in a manner related to the epitopes generated by the nonamyloidogenic processing of App. The presence of Vcp suggests a role for App-EVs in the clearance of protein aggregates.\n                <\/jats:p>","DOI":"10.1101\/2025.01.20.633888","type":"posted-content","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T18:20:19Z","timestamp":1737570019000},"source":"Crossref","is-referenced-by-count":1,"title":["Valosin-containing Protein is Cargo in Amyloid Precursor Protein Extracellular Vesicles"],"prefix":"10.64898","author":[{"given":"Yue","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Physiology & Neuroscience New Jersey Medical School, Brain Health Institute, Rutgers, The State University of New Jersey, 185 South Orange Ave, Newark, NJ, 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