{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:30:51Z","timestamp":1785429051010,"version":"3.56.0"},"posted":{"date-parts":[[2025,1,22]]},"group-title":"Evolutionary Biology","reference-count":47,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["2K12GM119955"],"award-info":[{"award-number":["2K12GM119955"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart Lung and Blood Institute","doi-asserted-by":"crossref","award":["HL121212"],"award-info":[{"award-number":["HL121212"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart Lung and Blood Institute","doi-asserted-by":"crossref","award":["HL171421"],"award-info":[{"award-number":["HL171421"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart Lung and Blood Institute","doi-asserted-by":"crossref","award":["HL150784"],"award-info":[{"award-number":["HL150784"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["DEB 2316784"],"award-info":[{"award-number":["DEB 2316784"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Interactions between predators and prey are often characterized by strong selection pressures that shape extreme physiological adaptations. Venom resistance in large-bodied South American opossums (Clade Didelphini) is a striking example, as these marsupials prey on venomous snakes and exhibit remarkable resistance to their venom. While resistance is well documented in Didelphini, relatively little is known about venom resistance in the smaller, more diverse members of Didelphidae, which inhabit the same regions and encounter the same predators. Here, we investigate venom resistance in the small-bodied opossum,\n                  <jats:italic>Monodelphis domestica<\/jats:italic>\n                  , through multi-level physiological assays, examining responses to purified venom components and whole venom from sympatric and allopatric vipers. Our results show\n                  <jats:italic>M. domestica<\/jats:italic>\n                  resists venom-induced disruptions to blood coagulation, retains platelet function in the presence of platelet-disrupting venoms, and inhibits snake venom metalloproteinases. Unexpectedly, we find that\n                  <jats:italic>M. domestica<\/jats:italic>\n                  von Willebrand Factor (VWF) requires increased shear force to elongate, a previously unknown aspect of opossum blood physiology that may contribute to venom resistance and may have relevance to human coagulopathies. These findings expand the extent of venom resistance beyond large-bodied Didelphini, suggesting it is a widespread trait in South American marsupials and providing new insights into venom-mammal coevolution.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Abstract Figure<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" fig-type=\"figure\" orientation=\"portrait\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"634112v2_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>","DOI":"10.1101\/2025.01.21.634112","type":"posted-content","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T17:25:18Z","timestamp":1737566718000},"source":"Crossref","is-referenced-by-count":0,"title":["Species-specific, multifaceted venom resistance in\n                  <i>Monodelphis domestica<\/i>\n                  reveals novel physiological behavior of von Willebrand Factor under flow"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3477-3012","authenticated-orcid":false,"given":"Matthew","family":"Holding","sequence":"first","affiliation":[{"name":"Life Sciences Institute, University of Michigan, Ann Arbor, MI"},{"name":"Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4896-674X","authenticated-orcid":false,"given":"Dante","family":"Disharoon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2237-659X","authenticated-orcid":false,"given":"Laura","family":"Haynes","sequence":"additional","affiliation":[{"name":"Life Sciences Institute, University of Michigan, Ann Arbor, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7285-023X","authenticated-orcid":false,"given":"Bipin Chakravarthy","family":"Paruchuri","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1826-4533","authenticated-orcid":false,"given":"M. Hao","family":"Hao Pontius","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1679-797X","authenticated-orcid":false,"given":"Krista","family":"Golden","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2874-4904","authenticated-orcid":false,"given":"Jordan A.","family":"Shavit","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA"},{"name":"Departments of Human Genetics, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0392-4033","authenticated-orcid":false,"given":"Karl","family":"Desch","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6436-8942","authenticated-orcid":false,"given":"David","family":"Ginsburg","sequence":"additional","affiliation":[{"name":"Life Sciences Institute, University of Michigan, Ann Arbor, MI"},{"name":"Department of Pediatrics, University of Michigan, Ann Arbor, MI, USA"},{"name":"Departments of Human Genetics, University of Michigan, Ann Arbor, MI, USA"},{"name":"Departments of Internal Medicine, University of Michigan, Ann Arbor, MI, US"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5773-0667","authenticated-orcid":false,"given":"Anirban Sen","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Cruz","sequence":"additional","affiliation":[{"name":"Oberlin College Department of Biology, Oberlin, OH 44074, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7096-3254","authenticated-orcid":false,"given":"Danielle H.","family":"Drabeck","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolution, and Behavior, University of Minnesota, Twin Cities"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041122073284000_2025.01.21.634112v2.1","first-page":"1","article-title":"Predation by the Opossunn Didelphis marsupialis on the Rattlesnake Crotalus durissus","volume":"19","year":"2000","journal-title":"Curr.Herpetol"},{"key":"2026041122073284000_2025.01.21.634112v2.2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444903021620"},{"key":"2026041122073284000_2025.01.21.634112v2.3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00198.x"},{"key":"2026041122073284000_2025.01.21.634112v2.4","unstructured":"Biardi, J. 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