{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:55:42Z","timestamp":1768517742623,"version":"3.49.0"},"posted":{"date-parts":[[2025,1,23]]},"group-title":"Developmental Biology","reference-count":28,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Diabetes is a condition characterized by a loss of pancreatic \u03b2\u2212cell function which results in the dysregulation of insulin homeostasis. Using a partial pancreatectomy model in axolotl, we aimed to observe the pancreatic response to injury.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Here we show a comprehensive histological assessment of pancreatic islets in axolotl. Following pancreatic injury, no apparent blastemal structure was observed. We found a significant, organ-wide increase in cellular proliferation post-resection in the pancreas compared to sham-operated controls. This proliferative response was most robust at the site of injury. We found that \u03b2\u2212cells actively contributed to the increased rates of proliferation upon injury. \u03b2\u2212cell proliferation manifested in increased \u03b2\u2212cell mass in injured tissue at two weeks post injury. At four weeks post injury, we found organ-wide proliferation to be extinguished while proliferation at the injury site persisted, corresponding to pancreatic tissue recovery. Similarly, total \u03b2\u2212cell mass was comparable to sham after four weeks.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings suggest a non-blastema-mediated regeneration process takes place in the pancreas, by which pancreatic resection induces whole-organ \u03b2\u2212cell proliferation without the formation of a blastemal structure. This process is analogous to other models of compensatory growth in axolotl, including liver regeneration.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.01.23.634564","type":"posted-content","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T23:20:14Z","timestamp":1737674414000},"source":"Crossref","is-referenced-by-count":0,"title":["Pancreatic injury induces \u03b2\u2212cell regeneration in axolotl"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6889-0099","authenticated-orcid":false,"given":"Connor J.","family":"Powell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7722-2758","authenticated-orcid":false,"given":"Hani D.","family":"Singer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley R.","family":"Juarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan T.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3717-0244","authenticated-orcid":false,"given":"Duygu","family":"Payzin-Dogru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2636-6145","authenticated-orcid":false,"given":"Aaron M.","family":"Savage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7005-298X","authenticated-orcid":false,"given":"Noah J.","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4297-9363","authenticated-orcid":false,"given":"Steven J.","family":"Blair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8811-0800","authenticated-orcid":false,"given":"Adnan","family":"Abouelela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1793-8106","authenticated-orcid":false,"given":"Anita","family":"Dittrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8008-2486","authenticated-orcid":false,"given":"Stuart G.","family":"Akeson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4571-3982","authenticated-orcid":false,"given":"Miten","family":"Jain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3709-6515","authenticated-orcid":false,"given":"Jessica L.","family":"Whited","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025012715250909000_2025.01.23.634564v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(20)30925-9"},{"key":"2025012715250909000_2025.01.23.634564v1.2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318172"},{"key":"2025012715250909000_2025.01.23.634564v1.3","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1144-0"},{"key":"2025012715250909000_2025.01.23.634564v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0088-0"},{"key":"2025012715250909000_2025.01.23.634564v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4030"},{"key":"2025012715250909000_2025.01.23.634564v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.09.004"},{"key":"2025012715250909000_2025.01.23.634564v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-024-00662-3"},{"key":"2025012715250909000_2025.01.23.634564v1.8","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110910"},{"key":"2025012715250909000_2025.01.23.634564v1.9","doi-asserted-by":"publisher","DOI":"10.2337\/db08-0628"},{"key":"2025012715250909000_2025.01.23.634564v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/nature02520"},{"key":"2025012715250909000_2025.01.23.634564v1.11","unstructured":"Dum\u00e9ril, A. 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