{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:57:07Z","timestamp":1785427027399,"version":"3.56.0"},"posted":{"date-parts":[[2025,1,29]]},"group-title":"Cell Biology","reference-count":85,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:00:00Z","timestamp":1738108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"European Research Council","award":["MemLessInterface (101078172)"],"award-info":[{"award-number":["MemLessInterface (101078172)"]}]},{"name":"Horizons Europe MSCA Doctoral Network Grant","award":["101167843 - BICEPS"],"award-info":[{"award-number":["101167843 - BICEPS"]}]},{"DOI":"10.13039\/100000181","name":"United States Air Force Office of Scientific Research","doi-asserted-by":"crossref","award":["FA9550-20-1-0241"],"award-info":[{"award-number":["FA9550-20-1-0241"]}],"id":[{"id":"10.13039\/100000181","id-type":"DOI","asserted-by":"crossref"}]},{"award":["7R01GM081506-13"],"award-info":[{"award-number":["7R01GM081506-13"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015326","name":"NIH Common Fund","doi-asserted-by":"crossref","award":["1R35GM156800"],"award-info":[{"award-number":["1R35GM156800"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015326","name":"NIH Common Fund","doi-asserted-by":"crossref","award":["F32 GM151858-02"],"award-info":[{"award-number":["F32 GM151858-02"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Condensates at synapses organize synaptic vesicle (SV) clusters and are essential for efficient neurotransmitter release. While it is established that RNA granules traffic along the axons, the function of RNA at the presynapse remains unclear. Here, we uncover a direct structural role of coding RNAs in organizing presynaptic condensates by focusing on SV clusters, condensates between synapsin-1 and lipid vesicles, a defining feature of nerve terminals. Using in vitro reconstitution systems, we show that RNA drives synapsin-1 coacervation, with bias toward structured RNAs being more effective at promoting phase transitions. The importance of RNA was confirmed in living synapses, where acute disruption of native RNA induces a dispersion of SVs and synapsin. Conversely, ectopically expressed SV-like condensates have the ability to recruit the translational machinery. The microscopy-based in vitro translation assay demonstrates increased translation efficiency within synapsin-1\/RNA condensates. Together, our work indicates a novel structural role of RNAs in modulating SV condensates.<\/jats:p>","DOI":"10.1101\/2025.01.29.635107","type":"posted-content","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T18:15:18Z","timestamp":1738174518000},"source":"Crossref","is-referenced-by-count":1,"title":["RNA promotes synapsin phase separation providing a platform for local translation"],"prefix":"10.64898","author":[{"given":"Branislava","family":"Rankovic","sequence":"first","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"},{"name":"Institute of Biochemistry, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health & Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117 Berlin, Germany"},{"name":"Institute of Biology, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary M","family":"Geisterfer","sequence":"additional","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"},{"name":"Department of Cell Biology, Duke University, Durham, North Carolina 27708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshita","family":"Chhabra","sequence":"additional","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"},{"name":"Institute of Biochemistry, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health & Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir M","family":"Jovanovic","sequence":"additional","affiliation":[{"name":"Human Biology and Primate Evolution, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Seim","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University, Durham, North Carolina 27708, USA"},{"name":"Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"},{"name":"Institute of Biochemistry, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health & Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonela","family":"Condric","sequence":"additional","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Aguilar P\u00e9rez","sequence":"additional","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinse","family":"de Boer","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9147 Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars T.","family":"Hofstede","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9147 Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiara","family":"Freitag","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Nowick","sequence":"additional","affiliation":[{"name":"Human Biology and Primate Evolution, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Jendrach","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9816-8917","authenticated-orcid":false,"given":"Frank","family":"Heppner","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Despic","sequence":"additional","affiliation":[{"name":"Goethe University Frankfurt, Institute for Molecular Biosciences, Max-von-Laue-Str. 13, 60438 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"M\u00fcller-McNicoll","sequence":"additional","affiliation":[{"name":"Goethe University Frankfurt, Institute for Molecular Biosciences, Max-von-Laue-Str. 13, 60438 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert","family":"van den Bogaart","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9147 Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4847-4144","authenticated-orcid":false,"given":"Sofiia","family":"Reshetniak","sequence":"additional","affiliation":[{"name":"Institute for Neuro- and Sensory Physiology, University Medical Center G\u00f6ttingen, 37073, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy S.","family":"Gladfelter","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health & Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117 Berlin, Germany"},{"name":"Department of Cell Biology, Duke University, Durham, North Carolina 27708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6625-1879","authenticated-orcid":false,"given":"Dragomir","family":"Milovanovic","sequence":"additional","affiliation":[{"name":"Whitman Center, Marine Biological Laboratory, 02543 Woods Hole, MA, USA"},{"name":"Institute of Biochemistry, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health & Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117 Berlin, Germany"},{"name":"Einstein Center for Neuroscience, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041023132928000_2025.01.29.635107v2.1","doi-asserted-by":"crossref","unstructured":"R. Jahn , D. C. Cafiso , L. K. Tamm , Nat. Rev. Mol. Cell Biol. 2024, 25, 101.","DOI":"10.1038\/s41580-023-00668-x"},{"key":"2026041023132928000_2025.01.29.635107v2.2","doi-asserted-by":"crossref","unstructured":"V. Rangaraju , S. tom Dieck , E. M. Schuman , EMBO Rep. 2017, 18, 693.","DOI":"10.15252\/embr.201744045"},{"key":"2026041023132928000_2025.01.29.635107v2.3","doi-asserted-by":"crossref","unstructured":"C. E. Holt , K. C. Martin , E. M. Schuman , Nat. Struct. Mol. Biol. 2019, 26, 557.","DOI":"10.1038\/s41594-019-0263-5"},{"key":"2026041023132928000_2025.01.29.635107v2.4","doi-asserted-by":"crossref","unstructured":"N. T. Ingolia , S. Ghaemmaghami , J. R. S. Newman , J. S. Weissman , Science 2009, 324, 218.","DOI":"10.1126\/science.1168978"},{"key":"2026041023132928000_2025.01.29.635107v2.5","doi-asserted-by":"crossref","unstructured":"B. Koltun , S. Ironi , N. Gershoni-Emek , I. Barrera , M. Hleihil , S. Nanguneri , R. Sasmal , S. S. Agasti , D. Nair , K. Rosenblum , Nucleic Acids Res. 2020, 48, e32.","DOI":"10.1093\/nar\/gkaa042"},{"key":"2026041023132928000_2025.01.29.635107v2.6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2113929118"},{"key":"2026041023132928000_2025.01.29.635107v2.7","doi-asserted-by":"crossref","unstructured":"D. C. Dieterich , J. J. L. Hodas , G. Gouzer , I. Y. Shadrin , J. T. Ngo , A. Triller , D. A. Tirrell , E. M. Schuman , Nat. Neurosci. 2010, 13, 897.","DOI":"10.1038\/nn.2580"},{"key":"2026041023132928000_2025.01.29.635107v2.8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2802-14.2015"},{"key":"2026041023132928000_2025.01.29.635107v2.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.59.4.1284"},{"key":"2026041023132928000_2025.01.29.635107v2.10","doi-asserted-by":"crossref","unstructured":"A. Giuditta , E. Menichini , C. P. Capano , M. Langella , R. Martin , E. Castigli , B. B. Kaplan , J. Neurosci. Res. 1991, 28, 18.","DOI":"10.1002\/jnr.490280103"},{"key":"2026041023132928000_2025.01.29.635107v2.11","doi-asserted-by":"crossref","unstructured":"I. J. Cajigas , G. Tushev , T. J. Will , S. tom Dieck , N. Fuerst , E. M. Schuman , Neuron 2012, 74, 453.","DOI":"10.1016\/j.neuron.2012.02.036"},{"key":"2026041023132928000_2025.01.29.635107v2.12","doi-asserted-by":"crossref","unstructured":"T. Shigeoka , H. Jung , J. Jung , B. Turner-Bridger , J. Ohk , J. Q. Lin , P. S. Amieux , C. E. Holt , Cell 2016, 166, 181.","DOI":"10.1016\/j.cell.2016.05.029"},{"key":"2026041023132928000_2025.01.29.635107v2.13","doi-asserted-by":"crossref","unstructured":"T. J. Younts , H. R. Monday , B. Dudok , M. E. Klein , B. A. Jordan , I. Katona , P. E. Castillo , Neuron 2016, 92, 479.","DOI":"10.1016\/j.neuron.2016.09.040"},{"key":"2026041023132928000_2025.01.29.635107v2.14","doi-asserted-by":"crossref","unstructured":"A.-S. Hafner , P. G. Donlin-Asp , B. Leitch , E. Herzog , E. M. Schuman , Science 2019, 364.","DOI":"10.1126\/science.aau3644"},{"key":"2026041023132928000_2025.01.29.635107v2.15","doi-asserted-by":"crossref","unstructured":"A. Biever , C. Glock , G. Tushev , E. Ciirdaeva , T. Dalmay , J. D. Langer , E. M. Schuman , Science 2020, 367.","DOI":"10.1126\/science.aay4991"},{"key":"2026041023132928000_2025.01.29.635107v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/nn1773"},{"key":"2026041023132928000_2025.01.29.635107v2.17","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4506"},{"key":"2026041023132928000_2025.01.29.635107v2.18","doi-asserted-by":"crossref","unstructured":"J.-M. Cioni , J. Q. Lin , A. V. Holtermann , M. Koppers , M. A. H. Jakobs , A. Azizi , B. Turner-Bridger , T. Shigeoka , K. Franze , W. A. Harris , C. E. Holt , Cell 2019, 176, 56.","DOI":"10.1016\/j.cell.2018.11.030"},{"key":"2026041023132928000_2025.01.29.635107v2.19","doi-asserted-by":"crossref","unstructured":"Y.-C. Liao , M. S. Fernandopulle , G. Wang , H. Choi , L. Hao , C. M. Drerup , R. Patel , S. Qamar , J. Nixon-Abell , Y. Shen , W. Meadows , M. Vendruscolo , T. P. J. Knowles , M. Nelson , M. A. Czekalska , G. Musteikyte , M. A. Gachechiladze , C. A. Stephens , H. A. Pasolli , L. R. Forrest , P. S. George-Hyslop , J. Lippincott-Schwartz , M. E. Ward , Cell 2019, 179, 147.","DOI":"10.1016\/j.cell.2019.08.050"},{"key":"2026041023132928000_2025.01.29.635107v2.20","doi-asserted-by":"crossref","unstructured":"S. F. Banani , H. O. Lee , A. A. Hyman , M. K. Rosen , Nat. Rev. Mol. Cell Biol. 2017, 18, 285 298.","DOI":"10.1038\/nrm.2017.7"},{"key":"2026041023132928000_2025.01.29.635107v2.21","doi-asserted-by":"crossref","unstructured":"G. A. Corbet , R. Parker , Cold Spring Harb. Symp. Quant. Biol. 2020, 84, 040329.","DOI":"10.1101\/sqb.2019.84.040329"},{"key":"2026041023132928000_2025.01.29.635107v2.22","doi-asserted-by":"crossref","unstructured":"F. Besse , A. Ephrussi , Nat. Rev. Mol. Cell Biol. 2008, 9, 971.","DOI":"10.1038\/nrm2548"},{"key":"2026041023132928000_2025.01.29.635107v2.23","doi-asserted-by":"crossref","unstructured":"G. Liao , L. Mingle , L. V. D. Water , G. Liu , Wiley Interdiscip. Rev.: RNA 2015, 6, 1.","DOI":"10.1002\/wrna.1265"},{"key":"2026041023132928000_2025.01.29.635107v2.24","doi-asserted-by":"crossref","unstructured":"C. Glock , M. Heum\u00fcller , E. M. Schuman , Curr Opin Neurobiol 2017, 45, 169.","DOI":"10.1016\/j.conb.2017.05.005"},{"key":"2026041023132928000_2025.01.29.635107v2.25","doi-asserted-by":"crossref","unstructured":"T. W. Han , M. Kato , S. Xie , L. C. Wu , H. Mirzaei , J. Pei , M. Chen , Y. Xie , J. Allen , G. Xiao , S. L. McKnight , Cell 2012, 149, 768 779.","DOI":"10.1016\/j.cell.2012.04.016"},{"key":"2026041023132928000_2025.01.29.635107v2.26","doi-asserted-by":"crossref","unstructured":"A. Molliex , J. Temirov , J. Lee , M. Coughlin , A. P. Kanagaraj , H. J. Kim , T. Mittag , J. P. Taylor , Cell 2015, 163, 123 133.","DOI":"10.1016\/j.cell.2015.09.015"},{"key":"2026041023132928000_2025.01.29.635107v2.27","doi-asserted-by":"crossref","unstructured":"S. Maharana , J. Wang , D. K. Papadopoulos , D. Richter , A. Pozniakovsky , I. Poser , M. Bickle , S. Rizk , J. Guill\u00e9n-Boixet , T. M. Franzmann , M. Jahnel , L. Marrone , Y.-T. Chang , J. Sterneckert , P. Tomancak , A. A. Hyman , S. Alberti , Science 2018, 360, 918.","DOI":"10.1126\/science.aar7366"},{"key":"2026041023132928000_2025.01.29.635107v2.28","doi-asserted-by":"crossref","unstructured":"M. S. Fernandopulle , J. Lippincott-Schwartz , M. E. Ward , Nat Neurosci 2021, 24, 622.","DOI":"10.1038\/s41593-020-00785-2"},{"key":"2026041023132928000_2025.01.29.635107v2.29","doi-asserted-by":"crossref","unstructured":"F. Trnka , C. Hoffmann , H. Wang , R. Sansevrino , B. Rankovic , B. R. Rost , D. Schmitz , H. B. Schmidt , D. Milovanovic , Frontiers Cell Dev. Biol. 2021, 9, 716919.","DOI":"10.3389\/fcell.2021.716919"},{"key":"2026041023132928000_2025.01.29.635107v2.30","unstructured":"R. Sansevrino , C. Hoffmann , D. Milovanovic , Trends Neurosci. 2023."},{"key":"2026041023132928000_2025.01.29.635107v2.31","doi-asserted-by":"crossref","unstructured":"C. M. Clemson , J. N. Hutchinson , S. A. Sara , A. W. Ensminger , A. H. Fox , A. Chess , J. B. Lawrence , Mol. Cell 2009, 33, 717.","DOI":"10.1016\/j.molcel.2009.01.026"},{"key":"2026041023132928000_2025.01.29.635107v2.32","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abo5578"},{"key":"2026041023132928000_2025.01.29.635107v2.33","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.7.1751"},{"key":"2026041023132928000_2025.01.29.635107v2.34","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2686411"},{"key":"2026041023132928000_2025.01.29.635107v2.35","doi-asserted-by":"crossref","unstructured":"E. M. Langdon , Y. Qiu , A. G. Niaki , G. A. McLaughlin , C. A. Weidmann , T. M. Gerbich , J. A. Smith , J. M. Crutchley , C. M. Termini , K. M. Weeks , S. Myong , A. S. Gladfelter , Science 2018, 360, 922.","DOI":"10.1126\/science.aar7432"},{"key":"2026041023132928000_2025.01.29.635107v2.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.11.010"},{"key":"2026041023132928000_2025.01.29.635107v2.37","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-024-01452-5"},{"key":"2026041023132928000_2025.01.29.635107v2.38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-52346-x"},{"key":"2026041023132928000_2025.01.29.635107v2.39","doi-asserted-by":"crossref","unstructured":"J. Pathak , E. Priyadarshini , K. Rawat , H. B. Bohidar , Adv. Colloid Interface Sci. 2017, 250, 40.","DOI":"10.1016\/j.cis.2017.10.006"},{"key":"2026041023132928000_2025.01.29.635107v2.40","doi-asserted-by":"publisher","DOI":"10.1126\/science.1252884"},{"key":"2026041023132928000_2025.01.29.635107v2.41","doi-asserted-by":"crossref","unstructured":"D. Milovanovic , Y. Wu , X. Bian , P. D. Camilli , Science 2018, 361, 604 607.","DOI":"10.1126\/science.aat5671"},{"key":"2026041023132928000_2025.01.29.635107v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42372-6"},{"key":"2026041023132928000_2025.01.29.635107v2.43","doi-asserted-by":"crossref","unstructured":"C. Hoffmann , K. M. Ruff , I. A. Edu , M. K. Shinn , J. V. Tromm , M. R. King , A. Pant , H. Ausserw\u00f6ger , J. R. Morgan , T. P. J. Knowles , R. V. Pappu , D. Milovanovic , J. Mol. Biol. 2025, 168987.","DOI":"10.1016\/j.jmb.2025.168987"},{"key":"2026041023132928000_2025.01.29.635107v2.44","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-024-01519-3"},{"key":"2026041023132928000_2025.01.29.635107v2.45","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1821038116"},{"key":"2026041023132928000_2025.01.29.635107v2.46","unstructured":"I. Seim , V. Zhang , A. P. Jalihal , B. M. Stormo , S. J. Cole , J. Ekena , H. T. Nguyen , D. Thirumalai , A. S. Gladfelter , bioRxiv 2024, 2024.12.11.627970."},{"key":"2026041023132928000_2025.01.29.635107v2.47","doi-asserted-by":"publisher","DOI":"10.1007\/BF00818163"},{"key":"2026041023132928000_2025.01.29.635107v2.48","doi-asserted-by":"publisher","DOI":"10.1186\/1748-7188-6-26"},{"key":"2026041023132928000_2025.01.29.635107v2.49","doi-asserted-by":"crossref","unstructured":"C. Hoffmann , R. Sansevrino , G. Morabito , C. Logan , R. M. Vabulas , A. Ulusoy , M. Ganzella , D. Milovanovic , J Mol Biol 2021, 166961.","DOI":"10.1016\/j.jmb.2021.166961"},{"key":"2026041023132928000_2025.01.29.635107v2.50","doi-asserted-by":"crossref","unstructured":"M. Sharma , J. Burr\u00e9 , Trends Neurosci 2023, 46, 153.","DOI":"10.1016\/j.tins.2022.11.007"},{"key":"2026041023132928000_2025.01.29.635107v2.51","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-021-02296-y"},{"key":"2026041023132928000_2025.01.29.635107v2.52","doi-asserted-by":"crossref","unstructured":"K. Berg , M. Folini , L. Prasmickaite , P. Selbo , A. Bonsted , B. Engesaeter , N. Zaffaroni , A. Weyergang , A. Dietzea , G. Maelandsmo , E. Wagner , O.-J. Norum , A. Hogset , Curr. Pharm. Biotechnol. 2007, 8, 362.","DOI":"10.2174\/138920107783018354"},{"key":"2026041023132928000_2025.01.29.635107v2.53","doi-asserted-by":"crossref","unstructured":"T. H. Soe , K. Watanabe , T. Ohtsuki , Molecules 2020, 26, 36.","DOI":"10.3390\/molecules26010036"},{"key":"2026041023132928000_2025.01.29.635107v2.54","doi-asserted-by":"crossref","unstructured":"N. Neckelmann , C. K. Warner , A. Chung , J. Kudoh , S. Minoshima , R. Fukuyama , M. Maekawa , Y. Shimizu , N. Shimizu , J. D. Liu , D. C. Wallace , Genomics 1989, 5, 829.","DOI":"10.1016\/0888-7543(89)90125-0"},{"key":"2026041023132928000_2025.01.29.635107v2.55","doi-asserted-by":"publisher","DOI":"10.1002\/WRNA.1590;CTYPE:STRING:JOURNAL"},{"key":"2026041023132928000_2025.01.29.635107v2.56","doi-asserted-by":"crossref","unstructured":"S. Boersma , D. Khuperkar , B. M. P. Verhagen , S. Sonneveld , J. B. Grimm , L. D. Lavis , M. E. Tanenbaum , Cell 2019, 178, 458.","DOI":"10.1016\/j.cell.2019.05.001"},{"key":"2026041023132928000_2025.01.29.635107v2.57","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-32245-9"},{"key":"2026041023132928000_2025.01.29.635107v2.58","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.05.008"},{"key":"2026041023132928000_2025.01.29.635107v2.59","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23188-8"},{"key":"2026041023132928000_2025.01.29.635107v2.60","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41525-022-00319-8","volume":"7","year":"2022","journal-title":"npj Genom. Med."},{"key":"2026041023132928000_2025.01.29.635107v2.61","doi-asserted-by":"crossref","unstructured":"O. Steward , W. Levy , J. Neurosci. 1982, 2, 284.","DOI":"10.1523\/JNEUROSCI.02-03-00284.1982"},{"key":"2026041023132928000_2025.01.29.635107v2.62","doi-asserted-by":"crossref","unstructured":"B. C. Yoon , H. Jung , A. Dwivedy , C. M. O\u2019Hare , K. H. Zivraj , C. E. Holt , Cell 2012, 148, 752.","DOI":"10.1016\/j.cell.2011.11.064"},{"key":"2026041023132928000_2025.01.29.635107v2.63","doi-asserted-by":"crossref","unstructured":"H. H.-W. Wong , A. J. Watt , P. J. Sj\u00f6str\u00f6m , Neuron 2024, 112, 264.","DOI":"10.1016\/j.neuron.2023.10.011"},{"key":"2026041023132928000_2025.01.29.635107v2.64","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.242487"},{"key":"2026041023132928000_2025.01.29.635107v2.65","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.02.027"},{"key":"2026041023132928000_2025.01.29.635107v2.66","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814385116"},{"key":"2026041023132928000_2025.01.29.635107v2.67","unstructured":"C. Hoffmann , G. Murastov , J. V. Tromm , J.-B. Moog , M. A. Aslam , A. Matkovic , D. Milovanovic , Nano Lett. 2023."},{"key":"2026041023132928000_2025.01.29.635107v2.68","doi-asserted-by":"publisher","DOI":"10.1016\/J.CHEMPR.2023.04.001"},{"key":"2026041023132928000_2025.01.29.635107v2.69","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35370-7"},{"key":"2026041023132928000_2025.01.29.635107v2.70","unstructured":"Z. M. Geisterfer , A. P. Jalihal , S. J. Cole , A. S. Gladfelter , bioRxiv 2024, 2024.12.12.628219."},{"key":"2026041023132928000_2025.01.29.635107v2.71","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1038\/s44318-025-00516-y","volume":"44","year":"2025","journal-title":"EMBO J."},{"key":"2026041023132928000_2025.01.29.635107v2.72","doi-asserted-by":"crossref","first-page":"ar10","DOI":"10.1091\/mbc.E23-07-0269","volume":"35","year":"2024","journal-title":"Mol. Biol. Cell"},{"key":"2026041023132928000_2025.01.29.635107v2.73","doi-asserted-by":"crossref","unstructured":"W. T. Snead , A. P. Jalihal , T. M. Gerbich , I. Seim , Z. Hu , A. S. Gladfelter , Nat. Cell Biol. 2022, 24, 461.","DOI":"10.1038\/s41556-022-00882-3"},{"key":"2026041023132928000_2025.01.29.635107v2.74","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac596"},{"key":"2026041023132928000_2025.01.29.635107v2.75","doi-asserted-by":"crossref","unstructured":"G. A. Banker , W. M. Cowan , Brain Res. 1977, 126, 397.","DOI":"10.1016\/0006-8993(77)90594-7"},{"key":"2026041023132928000_2025.01.29.635107v2.76","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.356"},{"key":"2026041023132928000_2025.01.29.635107v2.77","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020104596"},{"key":"2026041023132928000_2025.01.29.635107v2.78","doi-asserted-by":"crossref","unstructured":"A. Nagy , R. R. Baker , S. J. Morris , V. P. Whittaker , Brain Res 1976, 109, 285.","DOI":"10.1016\/0006-8993(76)90531-X"},{"key":"2026041023132928000_2025.01.29.635107v2.79","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.5.1374"},{"key":"2026041023132928000_2025.01.29.635107v2.80","doi-asserted-by":"crossref","unstructured":"S. Takamori , M. Holt , K. Stenius , E. A. Lemke , M. Gr\u00f8nborg , D. Riedel , H. Urlaub , S. Schenck , B. Br\u00fcgger , P. Ringler , S. A. M\u00fcller , B. Rammner , F. Gr\u00e4ter , J. S. Hub , B. L. D. Groot , G. Mieskes , Y. Moriyama , J. Klingauf , H. Grubm\u00fcller , J. Heuser , F. Wieland , R. Jahn , Cell 2006, 127, 831 846.","DOI":"10.1016\/j.cell.2006.10.030"},{"key":"2026041023132928000_2025.01.29.635107v2.81","first-page":"1180","volume":"59","year":"1999","journal-title":"Cancer Res."},{"key":"2026041023132928000_2025.01.29.635107v2.82","doi-asserted-by":"publisher","DOI":"10.1261\/rna.061184.117"},{"key":"2026041023132928000_2025.01.29.635107v2.83","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2370"},{"key":"2026041023132928000_2025.01.29.635107v2.84","unstructured":"S. Mendonsa , N. von K\u00fcgelgen , S. Dantsuji , M. Ron , L. Breimann , A. Baranovskii , I. L\u00f6dige , M. Kirchner , M. Fischer , N. Zerna , L. Bujanic , P. Mertins , I. Ulitsky , M. Chekulaeva , Nat. Neurosci. 2023, 26, 394."},{"key":"2026041023132928000_2025.01.29.635107v2.85","doi-asserted-by":"crossref","unstructured":"J. Schindelin , I. Arganda-Carreras , E. Frise , V. Kaynig , M. Longair , T. Pietzsch , S. Preibisch , C. Rueden , S. Saalfeld , B. Schmid , J.-Y. Tinevez , D. J. White , V. Hartenstein , K. Eliceiri , P. Tomancak , A. Cardona , Nat Methods 2012, 9, 676.","DOI":"10.1038\/nmeth.2019"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.01.29.635107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T06:13:48Z","timestamp":1775888028000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.01.29.635107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,29]]},"references-count":85,"URL":"https:\/\/doi.org\/10.1101\/2025.01.29.635107","relation":{},"subject":[],"published":{"date-parts":[[2025,1,29]]},"subtype":"preprint"}}