{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:52:36Z","timestamp":1791517956247,"version":"4.3.4"},"posted":{"date-parts":[[2025,2,3]]},"group-title":"Microbiology","reference-count":88,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,2,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The digestive tract represents the most complex interface of an organism with its biotope. Food may be contaminated by pathogens and toxicants while an abundant and complex microbiota thrives in the gut lumen. The organism must defend itself against potentially noxious biotic or abiotic stresses while preserving its microbiota, provided it plays a beneficial role. The presence of intestinal viruses adds another layer of complexity. Starting from a differential sensitivity of two lines from the same\n                  <jats:italic>Drosophila<\/jats:italic>\n                  wild-type strain to ingested\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  , we report here that the presence of Nora virus in the gut epithelium promotes the sensitivity to this bacterial pathogen as well as to an ingested oxidizing xenobiotic. The genotype, age, nature of the ingested food and, to a limited extent, the microbiota are relevant parameters that influence the effects of Nora virus on host fitness. Mechanistically, we detect the initial presence of the virus essentially in progenitor cells. Upon stress such as infection, exposure to xenobiotics, aging or feeding on a rich-food diet, the virus is then detected in enterocytes, which correlates with a disruption of the intestinal barrier function in aged flies. Finally, we show that the virus proliferates only when ISCs are induced to divide. We propose that enterocytes essentially get infected through lineage from progenitor cells and are not directly infected.\n                <\/jats:p>\n                <jats:p>In conclusion, it is important to check that experimental strains are devoid of intestinal viruses when monitoring survival\/life span of fly lines or when investigating the homeostasis of the intestinal epithelium as these viruses can constitute significant confounding factors.<\/jats:p>","DOI":"10.1101\/2025.01.30.635658","type":"posted-content","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T15:20:42Z","timestamp":1738596042000},"source":"Crossref","is-referenced-by-count":7,"title":["Nora virus proliferates in dividing intestinal stem cells and thereby sensitizes\n                  <i>Drosophila<\/i>\n                  flies to\n                  <i>Pseudomonas aeruginosa<\/i>\n                  intestinal infection and to oxidative stress"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8017-9260","authenticated-orcid":false,"given":"Adrien","family":"Franchet","sequence":"first","affiliation":[{"name":"UPR 9022 CNRS, IBMC, University of Strasbourg, France"},{"name":"Universit\u00e9 Bourgogne Europe, Institut Agro, CNRS, INRAE, UMR CSGA, 21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Haller","sequence":"additional","affiliation":[{"name":"UPR 9022 CNRS, IBMC, University of Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Yamba","sequence":"additional","affiliation":[{"name":"UPR 9022 CNRS, IBMC, University of Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Barbier","sequence":"additional","affiliation":[{"name":"UPR 9022 CNRS, IBMC, University of Strasbourg, 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