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In the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  circadian clock network, four pairs of small ventral lateral neurons (sLN\n                  <jats:sub>v<\/jats:sub>\n                  s) serve as critical pacemakers. Peptidergic communication via sLN\n                  <jats:sub>v<\/jats:sub>\n                  release of the key output neuropeptide\n                  <jats:italic>Pigment Dispersing Factor<\/jats:italic>\n                  (PDF) has been well characterized. In contrast, little is known about the role of the synaptic connections that sLN\n                  <jats:sub>v<\/jats:sub>\n                  s form with downstream neurons. Connectomic analyses revealed that the sLN\n                  <jats:sub>v<\/jats:sub>\n                  s form strong synaptic connections with a group of previously uncharacterized neurons, SLP316. Here, we show that silencing synaptic output in the SLP316 neurons via tetanus toxin (TNT) expression shortens the free-running period, whereas hyper-exciting them by expressing the Na[+] channel NaChBac results in period lengthening. Under light-dark cycles, silencing SLP316 neurons also causes lower daytime activity and higher daytime sleep. Our results revealed that the main postsynaptic partners of the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  pacemaker neurons are a non-clock neuronal cell type that regulates the timing of sleep and activity.\n                <\/jats:p>","DOI":"10.1101\/2025.01.30.635801","type":"posted-content","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T13:50:30Z","timestamp":1738331430000},"source":"Crossref","is-referenced-by-count":0,"title":["Synaptic Targets of Circadian Clock Neurons Influence Core Clock Parameters"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2349-6585","authenticated-orcid":false,"given":"Eva","family":"Scholz-Carlson","sequence":"first","affiliation":[{"name":"Department of Biology, Indiana University Bloomington. Bloomington, 47401 IN"},{"name":"Department of Neuroscience and Behavior, Barnard College. 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