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Towards this end, we pursued Adenoviral vectors genetically modified with the \u201cDogTag\u201d molecular glue peptide, which forms a spontaneous covalent bond with its partner protein, \u201cDogCatcher\u201d. Genetic fusion of DogCatcher to single-domain or single-chain antibodies allowed covalent tethering of the antibody at defined locales on the vector capsid. This modification allowed simple, effective and exclusive targeting of the vector to cells bound by the linked antibody. This dramatically enhanced gene transfer into primary B and T cells\n                  <jats:italic>in vitro<\/jats:italic>\n                  and\n                  <jats:italic>in vivo<\/jats:italic>\n                  in mice. These studies form the basis of a novel method for targeting Adenovirus that is functional in stringent\n                  <jats:italic>in vivo<\/jats:italic>\n                  contexts and can be combined with additional well characterized Adenovirus modifications towards applications in cell engineering, gene therapy, vaccines, oncolytics, and others.\n                <\/jats:p>","DOI":"10.1101\/2025.01.31.635969","type":"posted-content","created":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T22:50:19Z","timestamp":1738450219000},"source":"Crossref","is-referenced-by-count":0,"title":["In vivo targeted gene delivery using Adenovirus-antibody molecular glue conjugates"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7378-4940","authenticated-orcid":false,"given":"Paul J.","family":"Rice-Boucher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena A.","family":"Kashentseva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9206-1975","authenticated-orcid":false,"given":"Igor P.","family":"Dmitriev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongjie","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline M.","family":"Tremblay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9738-1361","authenticated-orcid":false,"given":"Charles B.","family":"Shoemaker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David T.","family":"Curiel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi Hong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025020400251315000_2025.01.31.635969v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00487-6"},{"key":"2025020400251315000_2025.01.31.635969v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-018-0039-1"},{"key":"2025020400251315000_2025.01.31.635969v1.3","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan4672"},{"key":"2025020400251315000_2025.01.31.635969v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.023"},{"key":"2025020400251315000_2025.01.31.635969v1.5","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00106"},{"key":"2025020400251315000_2025.01.31.635969v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41417-019-0157-z"},{"key":"2025020400251315000_2025.01.31.635969v1.7","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13668"},{"key":"2025020400251315000_2025.01.31.635969v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-02085-z"},{"key":"2025020400251315000_2025.01.31.635969v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01328-9"},{"key":"2025020400251315000_2025.01.31.635969v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2020.05.005"},{"key":"2025020400251315000_2025.01.31.635969v1.11","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2019.1671761"},{"key":"2025020400251315000_2025.01.31.635969v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2022.06.004"},{"key":"2025020400251315000_2025.01.31.635969v1.13","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2022-006292"},{"key":"2025020400251315000_2025.01.31.635969v1.14","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201809158"},{"key":"2025020400251315000_2025.01.31.635969v1.15","doi-asserted-by":"publisher","DOI":"10.1126\/science.abm0594"},{"key":"2025020400251315000_2025.01.31.635969v1.16","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10060201"},{"key":"2025020400251315000_2025.01.31.635969v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2022.01.034"},{"key":"2025020400251315000_2025.01.31.635969v1.18","doi-asserted-by":"crossref","unstructured":"Beatty, S. 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