{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:52:41Z","timestamp":1768521161734,"version":"3.49.0"},"posted":{"date-parts":[[2025,2,9]]},"group-title":"Microbiology","reference-count":66,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,2,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  is a Gram-negative opportunistic pathogen able to cause life- and sight-threating infections. Once considered an extracellular pathogen, numerous studies have shown it can survive intracellularly. Previously, we showed that\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  inside cells can diversify into distinct subpopulations in vacuoles and the cytoplasm. Here, we report that the transition from vacuoles to cytoplasm requires collaboration with the extracellular subpopulation, through Ca\n                  <jats:sup>2+<\/jats:sup>\n                  influx enabled by their type III secretion system (T3SS) translocon pore proteins. Moreover, we show that collaboration among\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  subpopulations can contribute to disseminating intracellular bacteria\n                  <jats:italic>in vivo<\/jats:italic>\n                  in a mouse infection model. This study provides the basis for future studies to investigate how cooperation of extracellular and intracellular bacteria within the host may contribute to disease progression and persistence.\n                <\/jats:p>","DOI":"10.1101\/2025.02.09.637186","type":"posted-content","created":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T23:20:16Z","timestamp":1739143216000},"source":"Crossref","is-referenced-by-count":1,"title":["Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis"],"prefix":"10.64898","author":[{"given":"Daniel","family":"Schator","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naren G","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel Joseph U","family":"Chong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy K","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Jedel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin E","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6885-2053","authenticated-orcid":false,"given":"David J","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6324-3462","authenticated-orcid":false,"given":"Suzanne M J","family":"Fleiszig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025021210302074000_2025.02.09.637186v1.1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1093\/jpids\/piac052","article-title":"Opportunistic Pathogens in Cystic Fibrosis: Epidemiology and Pathogenesis of Lung Infection","volume":"11","year":"2022","journal-title":"J Pediatric Infect Dis Soc"},{"key":"2025021210302074000_2025.02.09.637186v1.2","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2009.024"},{"key":"2025021210302074000_2025.02.09.637186v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2019.100804"},{"key":"2025021210302074000_2025.02.09.637186v1.4","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11111383"},{"key":"2025021210302074000_2025.02.09.637186v1.5","first-page":"157","article-title":"Pseudomonas Skin Infection","volume":"12","year":"2012","journal-title":"American Journal of Clinical Dermatology"},{"key":"2025021210302074000_2025.02.09.637186v1.6","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.14631218"},{"key":"2025021210302074000_2025.02.09.637186v1.7","doi-asserted-by":"crossref","unstructured":"S. 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