{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T23:50:12Z","timestamp":1771372212364,"version":"3.50.1"},"posted":{"date-parts":[[2025,2,13]]},"group-title":"Microbiology","reference-count":58,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,2,13]]},"abstract":"<jats:p>\n                  Outbreaks of cholera are caused by the highly transmissive pathogen\n                  <jats:italic>Vibrio cholerae<\/jats:italic>\n                  . Here, a transposon screen revealed that inactivation of the\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  motility-linked gene\n                  <jats:italic>motV<\/jats:italic>\n                  increases infant mouse intestinal colonization. Compared to wild-type\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  , a \u0394\n                  <jats:italic>motV<\/jats:italic>\n                  mutant, which exhibits heightened motility in the form of constitutive straight swimming, localizes to the crypts earlier in infection and over a larger area of the small intestine. Aberrant localization of the mutant was associated with an increased number of\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  initiating infection, and elevated pathogen burden, diarrhea, and lethality. Moreover, the deletion of\n                  <jats:italic>motV<\/jats:italic>\n                  causes\n                  <jats:italic>V. cholerae<\/jats:italic>\n                  to transmit from infected suckling mice to na\u00efve littermates more efficiently. Even in the absence of cholera toxin, the \u0394\n                  <jats:italic>motV<\/jats:italic>\n                  mutant continues to transmit between animals, although less than in the presence of toxin, indicating that phenotypes other than cholera toxin-driven diarrhea contribute to transmission. Collectively, this work provides experimental evidence linking intra-animal bottlenecks, colonization, and disease to inter-animal transmission.\n                <\/jats:p>","DOI":"10.1101\/2025.02.12.637895","type":"posted-content","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T23:21:59Z","timestamp":1739488919000},"source":"Crossref","is-referenced-by-count":1,"title":["A connection between\n                  <i>Vibrio cholerae<\/i>\n                  motility and inter-animal transmission"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3019-2560","authenticated-orcid":false,"given":"Ian W","family":"Campbell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7085-4760","authenticated-orcid":false,"given":"Ruchika","family":"Dehinwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander A.","family":"Morano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7013-0932","authenticated-orcid":false,"given":"Katherine G.","family":"Dailey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0874-4234","authenticated-orcid":false,"given":"Franz G.","family":"Zingl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1843-7000","authenticated-orcid":false,"given":"Matthew K.","family":"Waldor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025021507000659000_2025.02.12.637895v1.1","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/S0140-6736(17)30559-7","article-title":"Cholera","volume":"390","year":"2017","journal-title":"The Lancet"},{"key":"2025021507000659000_2025.02.12.637895v1.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003314"},{"key":"2025021507000659000_2025.02.12.637895v1.3","doi-asserted-by":"crossref","unstructured":"Eisenberg, M. , Robertson, S. & Tien, J . 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