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While antiviral therapies for CMV are approved, these compounds are limited by their toxicity and inability to specifically target the latent reservoir or prevent reactivation. Herein we show that CMV reactivation in Kasumi-3 cells, a CD34\n                  <jats:sup>+<\/jats:sup>\n                  hematopoietic cell line, requires mitogen-activated protein kinase (MAPK) activation. Importantly, pharmacological inhibition of the MAPK signaling pathway, including MEK and ERK, restricts viral reactivation in Kasumi-3 cells. In sum, our findings show MAPK signaling is critical for CMV reactivation, revealing a potential avenue for therapeutic intervention to prevent viral reactivation and downstream pathogenesis that is often detrimental for immunosuppressed and immunocompromised patients.\n                <\/jats:p>","DOI":"10.1101\/2025.02.13.638080","type":"posted-content","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T22:45:16Z","timestamp":1739486716000},"source":"Crossref","is-referenced-by-count":0,"title":["Inhibition of MAPK signaling suppresses cytomegalovirus reactivation in CD34\n                  <sup>+<\/sup>\n                  Kasumi-3 cells"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2882-7785","authenticated-orcid":false,"given":"Vargab","family":"Baruah","sequence":"first","affiliation":[{"name":"Infection Biology; Sheikha Fatima bint Mubarak Global Center for Pathogen and Human Health Research, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195 USA"},{"name":"Case Comprehensive Cancer Center, Cleveland, OH 44106 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0919-2961","authenticated-orcid":false,"given":"Benjamin A.","family":"Krishna","sequence":"additional","affiliation":[{"name":"Infection Biology; Sheikha Fatima bint Mubarak Global Center for Pathogen and Human Health Research, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195 USA"},{"name":"Case Comprehensive Cancer Center, Cleveland, OH 44106 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Infection Biology; Sheikha Fatima bint Mubarak Global Center for Pathogen and Human Health Research, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195 USA"},{"name":"Case Comprehensive Cancer Center, Cleveland, OH 44106 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Qi","sequence":"additional","affiliation":[{"name":"Infection Biology; Sheikha Fatima bint Mubarak Global Center for Pathogen and Human Health Research, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195 USA"},{"name":"Case Comprehensive Cancer Center, Cleveland, OH 44106 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4943-3774","authenticated-orcid":false,"given":"Christine M.","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Infection Biology; Sheikha Fatima bint Mubarak Global Center for Pathogen and Human Health Research, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195 USA"},{"name":"Case Comprehensive Cancer Center, Cleveland, OH 44106 USA"},{"name":"Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland Clinic, Cleveland, OH 44195 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041109521455000_2025.02.13.638080v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2022.05.005"},{"key":"2026041109521455000_2025.02.13.638080v1.2","unstructured":"Akpan, U.S. & Pillarisetty, L.S. , 2024. 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