{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T17:49:24Z","timestamp":1775929764603,"version":"3.50.1"},"posted":{"date-parts":[[2025,2,17]]},"group-title":"Cell Biology","reference-count":64,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,2,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Hybrid incompatibility can lead to lethality and sterility of F1 hybrids, contributing to speciation. Here we found that female hybrids between\n                  <jats:italic>Mus musculus domesticus<\/jats:italic>\n                  and\n                  <jats:italic>Mus spicilegus<\/jats:italic>\n                  mice are sterile due to the failure of homologous chromosome separation in oocyte meiosis I, producing aneuploid eggs. This non-separation phenotype was driven by the mis- localization of the cohesin protector, SGO2, along the chromosome arms instead of its typical centromeric enrichment, resulting in cohesin over-protection. The upstream kinase, BUB1, showed a significantly higher activity in hybrid oocytes, explaining SGO2 mis-targeting along the chromosome arm. Higher BUB1 activity was not observed in mitosis, consistent with viable hybrid mice. Cohesion defects were also evident in hybrid mice from another genus,\n                  <jats:italic>Peromyscus<\/jats:italic>\n                  , wherein cohesin protection is weakened. Defective cohesion in oocytes is a leading cause of reduced fertility especially with advanced maternal age. Our work provides evidence that a major cause of human infertility may play a positive role in promoting mammalian speciation.\n                <\/jats:p>","DOI":"10.1101\/2025.02.16.638358","type":"posted-content","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T06:00:49Z","timestamp":1739772049000},"source":"Crossref","is-referenced-by-count":1,"title":["Hybrid female sterility due to cohesin protection errors in oocytes"],"prefix":"10.64898","author":[{"given":"Warif","family":"El Yakoubi","sequence":"first","affiliation":[{"name":"Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health; Bethesda, Maryland 20894, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Pan","sequence":"additional","affiliation":[{"name":"Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health; Bethesda, Maryland 20894, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8406-9333","authenticated-orcid":false,"given":"Takashi","family":"Akera","sequence":"additional","affiliation":[{"name":"Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health; Bethesda, Maryland 20894, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041110244000000_2025.02.16.638358v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2010.04.005"},{"key":"2026041110244000000_2025.02.16.638358v1.2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1163934"},{"key":"2026041110244000000_2025.02.16.638358v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2022.07.037"},{"key":"2026041110244000000_2025.02.16.638358v1.4","unstructured":"J. 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