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As the molecular mechanisms governing SAT remodeling in this disease are unclear, we performed single-nucleus RNA sequencing on paired control and affected SAT biopsies from patients with unilateral lymphedema. Lymphedema samples were characterized by expansion of SAA\n                  <jats:sup>+<\/jats:sup>\n                  adipocytes, pro-adipogenic stem cells, and proliferation of lymphatic capillaries. A\n                  <jats:italic>GRIA1<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  lymphedema-enriched stromal cell population expressing\n                  <jats:italic>VEGFC<\/jats:italic>\n                  ,\n                  <jats:italic>ADAMTS3<\/jats:italic>\n                  , and\n                  <jats:italic>CCBE1<\/jats:italic>\n                  was identified, suggesting an enhanced axis of communication between adipose stem and progenitor cells (ASPCs) and lymphatic endothelial cells. Furthermore, lymphedema ASPC-conditioned media promoted lymphatic endothelial tube elongation and proliferation\n                  <jats:italic>in vitro<\/jats:italic>\n                  . These findings indicate a critical role for ASPCs in regulating adipocyte differentiation and lymphatic vascular remodeling in lymphedema, and provide a valuable resource for better understanding this disease.\n                <\/jats:p>","DOI":"10.1101\/2025.02.18.638907","type":"posted-content","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T14:25:17Z","timestamp":1739975117000},"source":"Crossref","is-referenced-by-count":3,"title":["Single-nuclear transcriptomics of lymphedema-associated adipose reveals a pro-lymphangiogenic stromal cell population"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4321-3992","authenticated-orcid":false,"given":"Gregory P.","family":"Westcott","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margo P.","family":"Emont","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Gulko","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charissa","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Radiology, Beth Israel Deaconess Medical Center, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gopal","family":"Varma","sequence":"additional","affiliation":[{"name":"Department of Radiology, Beth Israel Deaconess Medical Center, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo L.","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Radiology, Beth Israel Deaconess Medical Center, Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliza","family":"O\u2019Donnell","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zinger Yang","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenle","family":"Liang","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"},{"name":"Broad Institute, Cambridge, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linus T.","family":"Tsai","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Padera","sequence":"additional","affiliation":[{"name":"Edwin L. 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