{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T12:50:39Z","timestamp":1775998239588,"version":"3.50.1"},"posted":{"date-parts":[[2025,2,25]]},"group-title":"Microbiology","reference-count":27,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"name":"NIH-NIAID","award":["R01AI139267"],"award-info":[{"award-number":["R01AI139267"]}]},{"name":"NIH-NIAID","award":["R43AI155211"],"award-info":[{"award-number":["R43AI155211"]}]},{"name":"NIH-NIAID","award":["R44AI167605"],"award-info":[{"award-number":["R44AI167605"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,4,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The efficacy and duration of passive immunity protection depends on maternal antibody levels and transfer efficiency. We investigated whether oral vaccination of\n                  <jats:italic>Peromyscus leucopus<\/jats:italic>\n                  dams with recombinant OspA-expressing\n                  <jats:italic>E. coli<\/jats:italic>\n                  could induce maternal transfer of anti-OspA antibodies and protect pups from\n                  <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                  challenge. Dams were vaccinated until breeding pairs were created (i), until parturition (ii), and until pups were 2 weeks old (iii). Pups were challenged with nymphal\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  -transmitted\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  at \u223c 4 weeks of age. Anti-OspA IgG were quantified in dams and pups, and anti\n                  <jats:italic>-B. burgdorferi<\/jats:italic>\n                  IgG were quantified in pups.\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  burden was assessed by\n                  <jats:italic>flaB<\/jats:italic>\n                  qPCR in pups\u2019 tissues \u223c 4 weeks after tick challenge and viability of\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  was assessed by culture of heart tissue.\n                  <jats:italic>P. leucopus<\/jats:italic>\n                  pups born to dams vaccinated until breeding had low serologic anti-OspA antibody and were not protected from tick transmitted\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  infection. However, when dams\u2019 vaccination extended until parturition and until pups were two weeks old, significant anti-OspA antibody transfer and protection from\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  infection occurred. This was evidenced by absence of antibody to\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  PepVF, absence of\n                  <jats:italic>B. burgdorferi flaB<\/jats:italic>\n                  DNA in heart and bladder tissues, and absence of\n                  <jats:italic>flaB<\/jats:italic>\n                  in culture from heart tissues from pups euthanized &gt;9 weeks after birth. We show that transfer of anti-OspA antibodies from vaccinated\n                  <jats:italic>P. leucopus<\/jats:italic>\n                  dams to offspring prevents tick transmission and infection dynamics of\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  in the major reservoir host of this spirochete in the United States.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>\n                    This study contributes to our understanding of how interventions based in reservoir targeted OspA vaccines designed to block transmission of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    from infected\n                    <jats:italic>I. scapularis<\/jats:italic>\n                    ticks may disrupt the enzootic cycle of this spirochete and reduce incidence of Lyme disease.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.02.24.639966","type":"posted-content","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T23:50:23Z","timestamp":1740527423000},"source":"Crossref","is-referenced-by-count":0,"title":["Maternal Transfer of Oral Vaccine Induced Anti-OspA Antibodies Protects\n                  <i>Peromyscus<\/i>\n                  spp Pups from Tick-Transmitted\n                  <i>Borrelia burgdorferi<\/i>"],"prefix":"10.64898","author":[{"given":"Jose F.","family":"Azevedo","sequence":"first","affiliation":[{"name":"University of Tennessee Health Science Center, Department of Microbiology, Immunology, and Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg","family":"Joyner","sequence":"additional","affiliation":[{"name":"University of Tennessee Health Science Center, Department of Microbiology, Immunology, and 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Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041205213059000_2025.02.24.639966v2.1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-009-0077-3"},{"key":"2026041205213059000_2025.02.24.639966v2.2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1985.34.355"},{"key":"2026041205213059000_2025.02.24.639966v2.3","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1987.36.92"},{"key":"2026041205213059000_2025.02.24.639966v2.4","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/27.4.646"},{"key":"2026041205213059000_2025.02.24.639966v2.5","doi-asserted-by":"publisher","DOI":"10.1089\/153036601750137705"},{"key":"2026041205213059000_2025.02.24.639966v2.6","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu005"},{"key":"2026041205213059000_2025.02.24.639966v2.7","first-page":"136","article-title":"Blocking pathogen transmission at the source: reservoir targeted OspA-based vaccines against Borrelia 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