{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:20:31Z","timestamp":1789539631102,"version":"build-2803163510"},"posted":{"date-parts":[[2025,3,6]]},"group-title":"Cell Biology","reference-count":51,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Reproductive aging in females is characterized by a decline in oocyte quantity and quality, as well as uterine and cervical dysfunction that contributes to infertility and pregnancy complications. To investigate mechanisms underlying reproductive aging, we explored the contribution of\n                  <jats:italic>Spag17<\/jats:italic>\n                  , a cilia-related gene associated with tissue homeostasis and fibrosis.\n                  <jats:italic>Spag17<\/jats:italic>\n                  was expressed throughout the female reproductive tract; however, its expression declined with age in ovarian tissue, while high expression levels were observed in the cervix of young females during cervical tissue remodeling in the pre- and post-parturition periods. Loss of\n                  <jats:italic>Spag17<\/jats:italic>\n                  in mice resulted in impaired fertility, obstructed labor, and maternal death. This phenotype was associated with accelerated ovarian aging, increased fibrosis, and cervical stiffness, further complicating parturition. At the molecular level,\n                  <jats:italic>Spag17<\/jats:italic>\n                  loss activated key aging-associated pathways, including proinflammatory, profibrotic, and senescence signaling, suggesting that SPAG17 may be a critical player in female reproductive aging.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>TEASER<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Spag17<\/jats:italic>\n                    is a key modulator of female reproductive aging.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.03.01.640648","type":"posted-content","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T09:55:17Z","timestamp":1741254917000},"source":"Crossref","is-referenced-by-count":1,"title":["Regulation of Female Reproductive Aging by the\n                  <i>Spag17<\/i>\n                  Gene"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4178-0853","authenticated-orcid":false,"given":"Valerie","family":"Ericsson","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madisyn","family":"Elam","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1986-6491","authenticated-orcid":false,"given":"Paulene","family":"Sapao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"},{"name":"Department of Chemistry, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le My Tu","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7807-4448","authenticated-orcid":false,"given":"Mary Ellen","family":"Gill","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saikeerthana","family":"Chodavarapu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0780-3004","authenticated-orcid":false,"given":"Carlos","family":"C\u00f3rdova-Fletes","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"},{"name":"Departamento de Bioqu\u00edmica y Medicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5986-2228","authenticated-orcid":false,"given":"Audrey","family":"Lafrenaye","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7584-6252","authenticated-orcid":false,"given":"Sonia","family":"Hassan","sequence":"additional","affiliation":[{"name":"Office of Women\u2019s Health, Wayne State University. Detroit, MI"},{"name":"Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI"},{"name":"Department of Physiology, Wayne State University School of Medicine, Detroit, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mala","family":"Mahendroo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3458-1830","authenticated-orcid":false,"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"PharmaNest Inc., Princeton, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3468-7114","authenticated-orcid":false,"given":"Mathieu","family":"Petitjean","sequence":"additional","affiliation":[{"name":"PharmaNest Inc., Princeton, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6199-0480","authenticated-orcid":false,"suffix":"III","given":"Jerome F","family":"Strauss","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8400-687X","authenticated-orcid":false,"given":"John","family":"Varga","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3756-9394","authenticated-orcid":false,"given":"Francesca E","family":"Duncan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7035-2202","authenticated-orcid":false,"given":"Maria E","family":"Teves","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041112143332000_2025.03.01.640648v1.1","doi-asserted-by":"crossref","unstructured":"C. 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