{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:47:11Z","timestamp":1782280031911,"version":"3.54.5"},"posted":{"date-parts":[[2025,3,15]]},"group-title":"Cell Biology","reference-count":74,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:00:00Z","timestamp":1741996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Immune cells rapidly respond to tissue damage through dynamic properties of the cytoskeleton. How microtubules control immune cell functions during injury responses remains poorly understood. Within skin, tissue-resident macrophages known as Langerhans cells use dynamic dendrites to surveil the epidermis for damage and migrate through a densely packed epithelium to wounds. Here, we use Langerhans cells within the adult zebrafish epidermis as a model to investigate roles for microtubules in immune cell tissue surveillance, phagocytosis, and directed migration. We describe microtubule organization within Langerhans cells, and show that depolymerizing the microtubule cytoskeleton alters dendrite morphology, debris engulfment, and migration efficiency. We find that the microtubule organizing center positions adjacent to engulfed debris and that its position correlates with navigational pathfinding during directed cell migration. Stabilizing microtubules prevents Langerhans cell motility during directed cell migration by impairing navigation around cellular obstacles. Collectively, our work demonstrates requirements for microtubules in the dynamic actions of tissue-resident macrophages during epithelial surveillance and wound repair.<\/jats:p>","DOI":"10.1101\/2025.03.13.642867","type":"posted-content","created":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T05:10:16Z","timestamp":1742015416000},"source":"Crossref","is-referenced-by-count":0,"title":["Microtubule-dependent cell polarity regulates skin-resident macrophage phagocytosis and directed cell migration"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7635-6658","authenticated-orcid":false,"given":"Eric","family":"Peterman","sequence":"first","affiliation":[{"name":"Department of Biology, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian A.","family":"Swinburne","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9330-2934","authenticated-orcid":false,"given":"Sean G.","family":"Megason","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6997-3773","authenticated-orcid":false,"given":"Jeffrey P.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Washington, Seattle, Washington, USA"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, 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