{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:45:43Z","timestamp":1775979943679,"version":"3.50.1"},"posted":{"date-parts":[[2025,3,18]]},"group-title":"Neuroscience","reference-count":98,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Chronic neuroinflammation and misfolded \u03b1-synuclein (\u03b1Syn) have been identified as key pathological correlates driving Parkinson\u2019s disease (PD) pathogenesis; however, the contribution of ion channels to microglia activation in the context of \u03b1-synucleinopathy remains elusive. Herein, we show that KCa3.1, a calcium-activated potassium channel, is robustly upregulated within microglia in multiple preclinical models of PD and, most importantly, in human PD and dementia with Lewy bodies (DLB) brains. Pharmacological inhibition of KCa3.1 via senicapoc or TRAM-34 inhibits KCa3.1 channel activity and the associated reactive microglial phenotype in response to aggregated \u03b1Syn, as well as ameliorates of PD like pathology in diverse PD mouse models. Additionally, proteomic and transcriptomic profiling of microglia revealed that senicapoc ameliorates aggregated \u03b1Syn-induced, inflammation-associated pathways and dysregulated metabolism in primary microglial cells. Mechanistically, FYN kinase in a STAT1 dependent manner regulates KCa3.1 mediated the microglial reactive activation phenotype after \u03b1-synucleinopathy. Moreover, reduced neuroinflammation and subsequent PD-like neuropathology were observed in SYN AAV inoculated KCa3.1 knockout mice. Together, these findings suggest that KCa3.1 inhibition represents a novel therapeutic strategy for treating patients with PD and related \u03b1-synucleinopathies.<\/jats:p>","DOI":"10.1101\/2025.03.18.643982","type":"posted-content","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T17:35:17Z","timestamp":1742405717000},"source":"Crossref","is-referenced-by-count":1,"title":["KCa3.1 Contributes to Neuroinflammation and Nigral Dopaminergic Neurodegeneration in Experimental models of Parkinson\u2019s Disease"],"prefix":"10.64898","author":[{"given":"Manikandan","family":"Samidurai","sequence":"first","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karthick","family":"Chennakesavan","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Souvarish","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Parkinson\u2019s Disorder Research Program, Iowa Center for Advanced Neurotoxicology, Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emir","family":"Malovic","sequence":"additional","affiliation":[{"name":"Parkinson\u2019s Disorder Research Program, Iowa Center for Advanced Neurotoxicology, Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai M.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, University of California, Davis, Davis, CA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latika","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, University of California, Davis, Davis, CA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa","family":"Ealy","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chelva","family":"Janarthanam","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bharathi Niveditha","family":"Palanisamy","sequence":"additional","affiliation":[{"name":"Parkinson\u2019s Disorder Research Program, Iowa Center for Advanced Neurotoxicology, Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naveen","family":"Kondru","sequence":"additional","affiliation":[{"name":"Parkinson\u2019s Disorder Research Program, Iowa Center for Advanced Neurotoxicology, Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Zenitsky","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huajun","family":"Jin","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vellareddy","family":"Anantharam","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anumantha","family":"Kanthasamy","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"zhang","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heike","family":"Wulff","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, University of California, Davis, Davis, CA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthi","family":"Kanthasamy","sequence":"additional","affiliation":[{"name":"Isakson Center for Neurological Disease Research, Department of Physiology and Pharmacology, University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041200051542000_2025.03.18.643982v1.1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01310059"},{"key":"2026041200051542000_2025.03.18.643982v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/42166"},{"key":"2026041200051542000_2025.03.18.643982v1.3","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-021-01288-2"},{"key":"2026041200051542000_2025.03.18.643982v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70068-7"},{"key":"2026041200051542000_2025.03.18.643982v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2020.10.016"},{"key":"2026041200051542000_2025.03.18.643982v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2004.01.005"},{"key":"2026041200051542000_2025.03.18.643982v1.7","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-020-01083-5"},{"key":"2026041200051542000_2025.03.18.643982v1.8","doi-asserted-by":"publisher","DOI":"10.14802\/jmd.21078"},{"key":"2026041200051542000_2025.03.18.643982v1.9","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-021-00242-5"},{"key":"2026041200051542000_2025.03.18.643982v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2019.07.005"},{"key":"2026041200051542000_2025.03.18.643982v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15119-w"},{"key":"2026041200051542000_2025.03.18.643982v1.12","doi-asserted-by":"publisher","DOI":"10.1038\/nm1747"},{"key":"2026041200051542000_2025.03.18.643982v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/nm1746"},{"key":"2026041200051542000_2025.03.18.643982v1.14","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz104"},{"key":"2026041200051542000_2025.03.18.643982v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELL.2021.09.007"},{"key":"2026041200051542000_2025.03.18.643982v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05672-9"},{"key":"2026041200051542000_2025.03.18.643982v1.17","doi-asserted-by":"crossref","unstructured":"Isik, S. , et al., Microglia Mediated Neuroinflammation in Parkinson\u2019s Disease. Cells, 2023. 12(7).","DOI":"10.3390\/cells12071012"},{"key":"2026041200051542000_2025.03.18.643982v1.18","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.661505"},{"key":"2026041200051542000_2025.03.18.643982v1.19","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25767-1"},{"key":"2026041200051542000_2025.03.18.643982v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.09.044"},{"key":"2026041200051542000_2025.03.18.643982v1.21","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20182191"},{"key":"2026041200051542000_2025.03.18.643982v1.22","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-018-0276-2"},{"key":"2026041200051542000_2025.03.18.643982v1.23","doi-asserted-by":"publisher","DOI":"10.1586\/ecp.10.11"},{"key":"2026041200051542000_2025.03.18.643982v1.24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032414-112212"},{"key":"2026041200051542000_2025.03.18.643982v1.25","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X15666170630164226"},{"key":"2026041200051542000_2025.03.18.643982v1.26","first-page":"868972","article-title":"Microglial KCa3.1 Channels as a Potential Therapeutic Target for Alzheimer\u2019s Disease","volume":"2012","year":"2012","journal-title":"Int J Alzheimers Dis"},{"key":"2026041200051542000_2025.03.18.643982v1.27","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15611434"},{"key":"2026041200051542000_2025.03.18.643982v1.28","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-023-02030-2"},{"key":"2026041200051542000_2025.03.18.643982v1.29","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.135244"},{"key":"2026041200051542000_2025.03.18.643982v1.30","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.754"},{"key":"2026041200051542000_2025.03.18.643982v1.31","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6905"},{"key":"2026041200051542000_2025.03.18.643982v1.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113707"},{"key":"2026041200051542000_2025.03.18.643982v1.33","doi-asserted-by":"publisher","DOI":"10.1186\/sl2974-019-1636-8"},{"key":"2026041200051542000_2025.03.18.643982v1.34","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24149"},{"key":"2026041200051542000_2025.03.18.643982v1.35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4658-15.2016"},{"key":"2026041200051542000_2025.03.18.643982v1.36","doi-asserted-by":"crossref","unstructured":"Langley, M. , et al., Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, and Progressive Neurodegeneration in MitoPark Transgenic Mice. Antioxid Redox Signal, 2017.","DOI":"10.1089\/ars.2016.6905"},{"key":"2026041200051542000_2025.03.18.643982v1.37","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00033"},{"key":"2026041200051542000_2025.03.18.643982v1.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2018.05.002"},{"key":"2026041200051542000_2025.03.18.643982v1.39","doi-asserted-by":"crossref","unstructured":"Sarkar, S. , et al., Manganese activates NLRP3 inflammasome signaling and propagates exosomal release of ASC in microglial cells. Sci Signal, 2019. 12(563).","DOI":"10.1126\/scisignal.aat9900"},{"key":"2026041200051542000_2025.03.18.643982v1.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.051"},{"key":"2026041200051542000_2025.03.18.643982v1.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104561"},{"key":"2026041200051542000_2025.03.18.643982v1.42","doi-asserted-by":"crossref","unstructured":"Conte, C. , et al., Toll-like Receptor 4 Is Upregulated in Parkinson\u2019s Disease Patients and Co-Localizes with pSer129alphaSyn: A Possible Link with the Pathology. Cells, 2023. 12(10).","DOI":"10.3390\/cells12101368"},{"key":"2026041200051542000_2025.03.18.643982v1.43","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.719807"},{"key":"2026041200051542000_2025.03.18.643982v1.44","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20180653"},{"key":"2026041200051542000_2025.03.18.643982v1.45","doi-asserted-by":"publisher","DOI":"10.1038\/nature11588"},{"key":"2026041200051542000_2025.03.18.643982v1.46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118819119"},{"key":"2026041200051542000_2025.03.18.643982v1.47","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.308461"},{"key":"2026041200051542000_2025.03.18.643982v1.48","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0302-15.2015"},{"key":"2026041200051542000_2025.03.18.643982v1.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2019.113082"},{"key":"2026041200051542000_2025.03.18.643982v1.50","doi-asserted-by":"crossref","unstructured":"Lee, R.D. , et al., Repurposing the K(Ca)3.1 Blocker Senicapoc for Ischemic Stroke. Transl Stroke Res, 2023.","DOI":"10.1007\/s12975-023-01152-6"},{"key":"2026041200051542000_2025.03.18.643982v1.51","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2021.577758"},{"key":"2026041200051542000_2025.03.18.643982v1.52","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.03.025"},{"key":"2026041200051542000_2025.03.18.643982v1.53","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4852"},{"key":"2026041200051542000_2025.03.18.643982v1.54","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-020-02198-5"},{"key":"2026041200051542000_2025.03.18.643982v1.55","doi-asserted-by":"publisher","DOI":"10.14802\/jmd.19046"},{"key":"2026041200051542000_2025.03.18.643982v1.56","doi-asserted-by":"crossref","unstructured":"Verma, D.K. , et al., Alpha-Synuclein Preformed Fibrils Induce Cellular Senescence in Parkinson\u2019s Disease Models. Cells, 2021. 10(7).","DOI":"10.3390\/cells10071694"},{"key":"2026041200051542000_2025.03.18.643982v1.57","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26519-x"},{"key":"2026041200051542000_2025.03.18.643982v1.58","doi-asserted-by":"publisher","DOI":"10.1126\/science.1227157"},{"key":"2026041200051542000_2025.03.18.643982v1.59","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awab242"},{"key":"2026041200051542000_2025.03.18.643982v1.60","doi-asserted-by":"crossref","unstructured":"Gordon, R. , et al., Inflammasome inhibition prevents \u03b1-synuclein pathology and dopaminergic neurodegeneration in mice. Sci Transl Med, 2018. 10(465).","DOI":"10.1126\/scitranslmed.aah4066"},{"key":"2026041200051542000_2025.03.18.643982v1.61","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0b013e31818e5e99"},{"key":"2026041200051542000_2025.03.18.643982v1.62","doi-asserted-by":"publisher","DOI":"10.1172\/JCI30836"},{"key":"2026041200051542000_2025.03.18.643982v1.63","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-017-0452-6"},{"key":"2026041200051542000_2025.03.18.643982v1.64","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2022.01.021"},{"key":"2026041200051542000_2025.03.18.643982v1.65","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20182191"},{"key":"2026041200051542000_2025.03.18.643982v1.66","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.101"},{"key":"2026041200051542000_2025.03.18.643982v1.67","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-019-1682-2"},{"key":"2026041200051542000_2025.03.18.643982v1.68","doi-asserted-by":"publisher","DOI":"10.1111\/pedi.12396"},{"key":"2026041200051542000_2025.03.18.643982v1.69","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910133107"},{"key":"2026041200051542000_2025.03.18.643982v1.70","doi-asserted-by":"publisher","DOI":"10.1016\/j.jointm.2022.01.001"},{"key":"2026041200051542000_2025.03.18.643982v1.71","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0682-22.2022"},{"key":"2026041200051542000_2025.03.18.643982v1.72","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2024.08.002"},{"key":"2026041200051542000_2025.03.18.643982v1.73","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2021.05.013"},{"key":"2026041200051542000_2025.03.18.643982v1.74","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.631375"},{"key":"2026041200051542000_2025.03.18.643982v1.75","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2016.04.008"},{"key":"2026041200051542000_2025.03.18.643982v1.76","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4658-15.2016"},{"key":"2026041200051542000_2025.03.18.643982v1.77","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00707.x"},{"key":"2026041200051542000_2025.03.18.643982v1.78","doi-asserted-by":"crossref","unstructured":"Alspach, E. , D.M. Lussier , and R.D. Schreiber , Interferon gamma and Its Important Roles in Promoting and Inhibiting Spontaneous and Therapeutic Cancer Immunity. Cold Spring Harb Perspect Biol, 2019. 11(3).","DOI":"10.1101\/cshperspect.a028480"},{"key":"2026041200051542000_2025.03.18.643982v1.79","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.67"},{"key":"2026041200051542000_2025.03.18.643982v1.80","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801794105"},{"key":"2026041200051542000_2025.03.18.643982v1.81","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-020-00976-9"},{"key":"2026041200051542000_2025.03.18.643982v1.82","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199304000-00024"},{"key":"2026041200051542000_2025.03.18.643982v1.83","doi-asserted-by":"crossref","unstructured":"Elgueta, D. , et al., Analyzing the Parkinson\u2019s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human alpha-Synuclein. J Vis Exp, 2022(185).","DOI":"10.3791\/63313"},{"key":"2026041200051542000_2025.03.18.643982v1.84","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-017-0416-x"},{"key":"2026041200051542000_2025.03.18.643982v1.85","doi-asserted-by":"crossref","unstructured":"Sarkar, S. , et al., Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility. J Vis Exp, 2017(122).","DOI":"10.3791\/55364-v"},{"key":"2026041200051542000_2025.03.18.643982v1.86","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605208103"},{"key":"2026041200051542000_2025.03.18.643982v1.87","doi-asserted-by":"publisher","DOI":"10.3233\/jpd-191683"},{"key":"2026041200051542000_2025.03.18.643982v1.88","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113716"},{"key":"2026041200051542000_2025.03.18.643982v1.89","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.08.028"},{"key":"2026041200051542000_2025.03.18.643982v1.90","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-016-9650-4"},{"key":"2026041200051542000_2025.03.18.643982v1.91","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05277-w"},{"key":"2026041200051542000_2025.03.18.643982v1.92","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"2026041200051542000_2025.03.18.643982v1.93","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026041200051542000_2025.03.18.643982v1.94","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-241"},{"key":"2026041200051542000_2025.03.18.643982v1.95","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.444224"},{"key":"2026041200051542000_2025.03.18.643982v1.96","first-page":"52139","article-title":"Detection of in situ protein-protein complexes at the Drosophila larval neuromuscular junction using proximity ligation assay","volume":"95","year":"2015","journal-title":"J Vis Exp"},{"key":"2026041200051542000_2025.03.18.643982v1.97","first-page":"2 16 1","article-title":"Detection of Receptor Heteromerization Using In Situ Proximity Ligation Assay","volume":"75","year":"2016","journal-title":"Curr Protoc Pharmacol"},{"key":"2026041200051542000_2025.03.18.643982v1.98","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.592374"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.03.18.643982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:05:27Z","timestamp":1775977527000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.03.18.643982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,18]]},"references-count":98,"URL":"https:\/\/doi.org\/10.1101\/2025.03.18.643982","relation":{},"subject":[],"published":{"date-parts":[[2025,3,18]]},"subtype":"preprint"}}