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Genetic, pharmacologic and electric neuromodulation of nociceptor activity in freely moving non-transgenic animals has been shown to be challenging due to many factors including the immunogenicity of non-mammalian proteins, procedure invasiveness and poor temporal precision. Here, we introduce a magnetogenetic strategy that enables remote bidirectional regulation of nociceptor activity. Magnetogenetics utilizes a source of direct magnetic field (DMF) to control neuronal activity in cells that express an anti-ferritin nanobody-TRPV1 receptor fusion protein (Nb-Ft-TRPV1). In our study, AAV2retro-mediated delivery of an excitatory Nb-Ft-TRPV1 construct into the sciatic nerve of wild-type mice resulted in stable long-term transgene expression accompanied by significant reduction of mechanical withdrawal thresholds during DMF exposure, place aversion of the DMF zone and activity changes in the anterior cingulate (ACC) nucleus. Conversely, delivery of an inhibitory variant of the Nb-Ft-TRPV1 construct, engineered to gate chloride ions in response to DMF, led to reversed behavioral manifestations of mechanical allodynia and showed place preference for the DMF zone, suggestive of functional pain relief. Changes in DRG activity were confirmed by post-mortem levels, immediately following DMF exposure, of the activity-induced gene\n                  <jats:italic>cfos<\/jats:italic>\n                  , which increased with the excitatory construct in normal mice and decreased with the inhibitory construct in pain models Our study demonstrates that magnetogenetic channels can achieve long-term expression in the periphery without losing functionality, providing a stable gene therapy system for non-invasive, magnetic field regulation of pain-related neurons for research and potential clinical applications.\n                <\/jats:p>","DOI":"10.1101\/2025.03.18.644041","type":"posted-content","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T19:19:10Z","timestamp":1742411950000},"source":"Crossref","is-referenced-by-count":2,"title":["Non-invasive in vivo bidirectional magnetogenetic modulation of pain circuits"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0728-3352","authenticated-orcid":false,"given":"Aldana M.","family":"Antoniazzi","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7319-6794","authenticated-orcid":false,"given":"Santiago R.","family":"Unda","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofya","family":"Norman","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa E.","family":"Pomeranz","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Marongiu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Stanley","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA"},{"name":"Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Friedman","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Kaplitt","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041200094794000_2025.03.18.644041v2.1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001939"},{"key":"2026041200094794000_2025.03.18.644041v2.2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000282763.29778.59"},{"key":"2026041200094794000_2025.03.18.644041v2.3","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000454"},{"key":"2026041200094794000_2025.03.18.644041v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2013.11.013"},{"key":"2026041200094794000_2025.03.18.644041v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(14)70251-0"},{"key":"2026041200094794000_2025.03.18.644041v2.6","doi-asserted-by":"crossref","unstructured":"F. 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