{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:30:42Z","timestamp":1778128242508,"version":"3.51.4"},"posted":{"date-parts":[[2025,3,19]]},"group-title":"Immunology","reference-count":49,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,19]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>\n                  Mouse resident peritoneal macrophages, called large cavity macrophages (LCM), arise from embryonic progenitors that proliferate as mature, CD73\n                  <jats:sup>+<\/jats:sup>\n                  Gata6\n                  <jats:sup>+<\/jats:sup>\n                  tissue-specialized macrophages. After injury from irradiation or inflammation, monocytes are thought to replenish CD73\n                  <jats:sup>+<\/jats:sup>\n                  Gata6\n                  <jats:sup>+<\/jats:sup>\n                  LCMs through a CD73\n                  <jats:sup>-<\/jats:sup>\n                  LYVE1\n                  <jats:sup>+<\/jats:sup>\n                  LCM intermediate. Here, we show that CD73\n                  <jats:sup>-<\/jats:sup>\n                  LYVE1\n                  <jats:sup>+<\/jats:sup>\n                  LCMs indeed yield Gata6\n                  <jats:sup>+<\/jats:sup>\n                  CD73\n                  <jats:sup>+<\/jats:sup>\n                  LCMs through integrin-mediated interactions with mesothelial surfaces. CD73\n                  <jats:sup>-<\/jats:sup>\n                  LYVE1\n                  <jats:sup>+<\/jats:sup>\n                  LCM repopulation of the peritoneum was reliant upon and quantitatively proportional to recruited monocytes. Unexpectedly, fate mapping indicated that only \u223c10% of Gata6-dependent LCMs that repopulated the peritoneum after injury depended on the LYVE1\n                  <jats:sup>+<\/jats:sup>\n                  LCM stage. Further supporting nonoverlapping lifecycles of CD73\n                  <jats:sup>-<\/jats:sup>\n                  LYVE1\n                  <jats:sup>+<\/jats:sup>\n                  and CD73\n                  <jats:sup>+<\/jats:sup>\n                  Gata6\n                  <jats:sup>+<\/jats:sup>\n                  LCMs, in mice bearing a paucity of monocytes, Gata6\n                  <jats:sup>+<\/jats:sup>\n                  CD73\n                  <jats:sup>+<\/jats:sup>\n                  LCMs rebounded after ablative irradiation substantially more efficiently than their presumed LYVE1\n                  <jats:sup>+<\/jats:sup>\n                  or CD73\n                  <jats:sup>-<\/jats:sup>\n                  LCM upstream precursors. Thus, after inflammatory insult, two temporally parallel pathways, each generating distinct differentiation intermediates with varying dependencies on monocytes, contribute to the replenish hment of Gata6\n                  <jats:sup>+<\/jats:sup>\n                  resident peritoneal macrophages.\n                <\/jats:p>","DOI":"10.1101\/2025.03.19.644175","type":"posted-content","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T18:33:15Z","timestamp":1742581995000},"source":"Crossref","is-referenced-by-count":2,"title":["Tracing LYVE1\n                  <sup>+<\/sup>\n                  peritoneal fluid macrophages unveils two paths to resident macrophage repopulation with differing reliance on monocytes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3127-1343","authenticated-orcid":false,"given":"Alexandre","family":"Gallerand","sequence":"first","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jichang","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel L.","family":"Mintz","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"},{"name":"Biomedical Engineering Graduate Program"},{"name":"Medical Scientist Training Program"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"},{"name":"Medical Scientist Training Program"},{"name":"Division of Biology and Biomedical Sciences Graduate Program"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel D.","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandy M.","family":"Chan","sequence":"additional","affiliation":[{"name":"Division of Biology and Biomedical Sciences Graduate Program"},{"name":"Division of Cardiology, Department of Internal Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyler T.","family":"Harmon","sequence":"additional","affiliation":[{"name":"Medical Scientist Training Program"},{"name":"Division of Biology and Biomedical Sciences Graduate Program"},{"name":"Division of Cardiology, Department of Internal Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Pediatrics Washington University School of Medicine, St. Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher G.","family":"Huckstep","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siling","family":"Du","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"},{"name":"Division of Biology and Biomedical Sciences Graduate Program"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiantian","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Kipnis","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Department of Pathology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kory J.","family":"Lavine","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3884-1994","authenticated-orcid":false,"given":"Joel D.","family":"Schilling","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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