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A key challenge of the pancreas slice model has been efficient transgene delivery throughout the slice volume while maintaining viability and function. Here, we demonstrate a robust adenoviral gene delivery approach to transduce slices with CaMPARI2 and apply photoconverting light to permanently mark glucose-induced calcium activity across all islets. This approach demonstrates glucose responsive CaMPARI2 labeling that correlates with insulin secretion. Using this novel high-throughput approach, we examine the relationship between islet size and calcium response. Larger isolated islets exhibit greater CaMPARI2 photoconversion in high glucose, whereas no size-function correlation is observed in islets resident in live slices. We also observe that slices capture a substantially higher proportion of small islets than isolated islets. Integrating CaMPARI2 with live pancreas slice studies enables multiplexed analyses, linking functional readouts to spatial features.<\/jats:p>","DOI":"10.1101\/2025.03.21.644659","type":"posted-content","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T03:56:10Z","timestamp":1742961370000},"source":"Crossref","is-referenced-by-count":1,"title":["Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5690-4748","authenticated-orcid":false,"given":"Charles S.","family":"Lazimi","sequence":"first","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin E.","family":"Stis","sequence":"additional","affiliation":[{"name":"J. 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Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8817-6355","authenticated-orcid":false,"given":"Clayton E.","family":"Mathews","sequence":"additional","affiliation":[{"name":"Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, University of Florida, Gainesville, FL, United States"},{"name":"Department of Infectious Diseases and Immunology, University of Florida College of Veterinary Medicine, University of Florida, Gainesville, FL, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8666-805X","authenticated-orcid":false,"given":"Edward A.","family":"Phelps","sequence":"additional","affiliation":[{"name":"J. 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