{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:23:11Z","timestamp":1790878991909,"version":"4.1.0"},"posted":{"date-parts":[[2025,3,25]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":41,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>\n                    Malaria is a vector-borne parasitic disease that continues to be a global public health threat. Five different species of the genus\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    (\n                    <jats:italic>P. falciparum, P. malariae, P. ovale curtisi, P. ovale wallikeri<\/jats:italic>\n                    , and\n                    <jats:italic>P. vivax)<\/jats:italic>\n                    cause malaria in Sub-Saharan Africa. Previous cross-sectional surveys from 2013 and 2017 indicated the circulation of\n                    <jats:italic>P. vivax<\/jats:italic>\n                    in the West region of Cameroon, prompting an investigation into the prevalence of all non-falciparum malaria parasite species in this region.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    A cross-sectional facility-based study recruited 431 clinically suspected cases of malaria from three health centres in the West region of Cameroon in 2020. Socio-demographic, clinical data, and dried blood spots (DBS) were collected from all consenting patients. Parasite DNA was extracted from DBS for real-time PCR amplification of species-specific\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    18S rRNA for\n                    <jats:italic>P. falciparum, P. ovale, P. malariae, and P. vivax<\/jats:italic>\n                    . In addition,\n                    <jats:italic>P. ovale<\/jats:italic>\n                    was further sub-classified into\n                    <jats:italic>P. ovale curtisi<\/jats:italic>\n                    or\n                    <jats:italic>P. ovale wallikeri<\/jats:italic>\n                    . The prevalence of different species was measured.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    Among the 431 samples, the overall malaria prevalence was 54.8% (236\/431). Of these, 49.9% were infected with\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    , 4% with\n                    <jats:italic>P. ovale<\/jats:italic>\n                    , and 0.9% with\n                    <jats:italic>P. malariae<\/jats:italic>\n                    . No\n                    <jats:italic>P. vivax<\/jats:italic>\n                    was detected. Mixed infections were common, with 3.5% of the infections harbouring more than one\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species. A total of 5\n                    <jats:italic>P. ovale<\/jats:italic>\n                    and 1\n                    <jats:italic>P. malariae<\/jats:italic>\n                    mono-infections were detected. Of the 17\n                    <jats:italic>P. ovale<\/jats:italic>\n                    infections, 12 were successfully genotyped, with 6\n                    <jats:italic>P. ovale curtisi<\/jats:italic>\n                    , 3\n                    <jats:italic>P. ovale<\/jats:italic>\n                    wallikeri, and 3 mixture of the species.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>\n                    While falciparum remains the dominant malaria parasite species among acute febrile illness cases, non-falciparum malaria is also commonly found in Dschang, both as a co-infection with\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    and as mono-infections. Both subspecies of\n                    <jats:italic>P. ovale<\/jats:italic>\n                    are present in the region. Continued monitoring of non-falciparum species is needed for understanding malaria burden in West Cameroon.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.03.24.25324523","type":"posted-content","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T03:40:23Z","timestamp":1742960423000},"source":"Crossref","is-referenced-by-count":5,"title":["Distribution of non-falciparum malaria among symptomatic malaria patients in Dschang, West Region of Cameroon"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0638-5573","authenticated-orcid":false,"given":"Pacome V. 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