{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:20:23Z","timestamp":1791505223630,"version":"4.3.4"},"posted":{"date-parts":[[2025,3,26]]},"group-title":"Plant Biology","reference-count":42,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,26]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>Many plastidial isoprenoids, including diterpenes and photosynthesis-related isoprenoids such as carotenoids and chlorophylls, derive from C20 geranylgeranyl diphosphate (GGPP), produced by GGPP synthase (GGPPS) enzymes. Heterodimers of GGPPS and non-catalytic type I small subunit (SSU-I) proteins produce C10 geranyl diphosphate (GPP), the precursor of monoterpenes.<\/jats:p>\n                <jats:p>\n                  Three plastidial GGPPS isoforms, referred to as SlG1-3, are present in tomato (\n                  <jats:italic>Solanum lycopersicum<\/jats:italic>\n                  ). Here we explored their contribution to the production of volatile organic compounds (VOCs) of isoprenoid origin under normal conditions and in response to infection with\n                  <jats:italic>Pseudomonas syringae<\/jats:italic>\n                  pathovar\n                  <jats:italic>tomato<\/jats:italic>\n                  (\n                  <jats:italic>Pst<\/jats:italic>\n                  ).\n                <\/jats:p>\n                <jats:p>\n                  Edited lines lacking SlG2 showed a distinctive VOC profile compared to unedited (WT) plants and mutants impaired in SlG1 or SlG3. In particular, only\n                  <jats:italic>slg2<\/jats:italic>\n                  mutants showed constitutively increased levels of GPP-derived hydroxylated monoterpenes (HMTPs). Upon\n                  <jats:italic>Pst<\/jats:italic>\n                  infection,\n                  <jats:italic>slg2<\/jats:italic>\n                  plants accumulated higher levels of salicylic acid (SA) and exhibited increase resistance compared to WT controls, resulting in reduced levels of VOCs associated to cell death.\n                <\/jats:p>\n                <jats:p>\n                  Our findings suggest that SlG2 regulates GPP synthesis, potentially by specifically competing with other GGPPS isoforms for heterodimerization with SSU-I. Increased GPP production in\n                  <jats:italic>slg2<\/jats:italic>\n                  plants could lead to higher HMTPs levels, which may result in elevated SA content, and subsequently enhanced protection against bacterial infection.\n                <\/jats:p>","DOI":"10.1101\/2025.03.24.644926","type":"posted-content","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T02:59:44Z","timestamp":1743044384000},"source":"Crossref","is-referenced-by-count":2,"title":["Loss of tomato geranylgeranyl diphosphate synthase 2 increases monoterpenoid levels and enhances immune responses to bacterial infection"],"prefix":"10.64898","author":[{"given":"Julia","family":"P\u00e9rez-P\u00e9rez","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP), CSIC-UPV, Ciudad Polit\u00e9cnica de la Innovaci\u00f3n (CPI) 8 E, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Ezquerro","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP), CSIC-UPV, Ciudad Polit\u00e9cnica de la Innovaci\u00f3n (CPI) 8 E, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sooyeon","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology, Graduate School of Green-Bio Science, College of Life Sciences, Kyung Hee University, 17104 Yongin, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun-Hwa","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology, Graduate School of Green-Bio Science, College of Life Sciences, Kyung Hee University, 17104 Yongin, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9251-0160","authenticated-orcid":false,"given":"M\u00aa Pilar","family":"L\u00f3pez-Gresa","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP), CSIC-UPV, Ciudad Polit\u00e9cnica de la Innovaci\u00f3n (CPI) 8 E, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1280-2305","authenticated-orcid":false,"given":"Manuel","family":"Rodr\u00edguez-Concepci\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP), CSIC-UPV, Ciudad Polit\u00e9cnica de la Innovaci\u00f3n (CPI) 8 E, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1662-8084","authenticated-orcid":false,"given":"Purificaci\u00f3n","family":"Lis\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP), CSIC-UPV, Ciudad Polit\u00e9cnica de la Innovaci\u00f3n (CPI) 8 E, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041200551827000_2025.03.24.644926v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08416-7"},{"key":"2026041200551827000_2025.03.24.644926v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-006-0301-5"},{"key":"2026041200551827000_2025.03.24.644926v1.3","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0606-2_5"},{"key":"2026041200551827000_2025.03.24.644926v1.4","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17283"},{"key":"2026041200551827000_2025.03.24.644926v1.5","doi-asserted-by":"publisher","DOI":"10.1007\/s42994-021-00050-5"},{"key":"2026041200551827000_2025.03.24.644926v1.6","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-005-0109-8"},{"key":"2026041200551827000_2025.03.24.644926v1.7","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.70052"},{"key":"2026041200551827000_2025.03.24.644926v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2014.01.016"},{"key":"2026041200551827000_2025.03.24.644926v1.9","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.071738"},{"key":"2026041200551827000_2025.03.24.644926v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2015.05.001"},{"key":"2026041200551827000_2025.03.24.644926v1.11","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19109"},{"key":"2026041200551827000_2025.03.24.644926v1.12","doi-asserted-by":"publisher","DOI":"10.1104\/pp.114.243246"},{"key":"2026041200551827000_2025.03.24.644926v1.13","doi-asserted-by":"crossref","unstructured":"Farahbakhsh F , Massah A , Hamzehzarghani H , Yassaie M , Amjadi Z , El-Zaeddi H , Carbonell-Barrachina AA (2023) Comparative profiling of volatile organic compounds associated to temperature sensitive resistance to wheat streak mosaic virus (WSMV) in resistant and susceptible wheat cultivars at normal and elevated temperatures. 281: 153903. 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