{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:51:39Z","timestamp":1775980299292,"version":"3.50.1"},"posted":{"date-parts":[[2025,3,25]]},"group-title":"Molecular Biology","reference-count":87,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Acute respiratory distress syndrome (ARDS) is an often fatal critical illness where lung epithelial injury leads to intrapulmonary fluid accumulation. ARDS became widespread during the COVID-19 pandemic, motivating a renewed effort to understand the complex etiology of this disease. Rigorous prior work has implicated lung endothelial and epithelial injury in response to an insult such as bacterial infection; however, the impact of microorganisms found in other organs on ARDS remains unclear. Here, we use a combination of gnotobiotic mice, cell culture experiments, and re-analyses of a large metabolomics dataset from ARDS patients to reveal that gut bacteria impact lung cellular respiration by releasing metabolites that alter mitochondrial activity in lung epithelium. Colonization of germ-free mice with a complex gut microbiota stimulated lung mitochondrial gene expression. A single human gut bacterial species,\n                  <jats:italic>Bifidobacterium adolescentis,<\/jats:italic>\n                  was sufficient to replicate this effect, leading to a significant increase in mitochondrial membrane potential in lung epithelial cells. We then used genome sequencing and mass spectrometry to confirm that\n                  <jats:italic>B. adolescentis<\/jats:italic>\n                  produces\n                  <jats:italic>L<\/jats:italic>\n                  -lactate, which was sufficient to increase mitochondrial activity in lung epithelial cells. Finally, we found that serum lactate was significantly associated with disease severity in patients with ARDS from the Early Assessment of Renal and Lung Injury (EARLI) cohort. Together, these results emphasize the importance of more broadly characterizing the microbial etiology of ARDS and other lung diseases given the ability of gut bacterial metabolites to remotely control lung cellular respiration. Our discovery of a single bacteria-metabolite pair provides a\n                  <jats:italic>proof-of-concept<\/jats:italic>\n                  for systematically testing other microbial metabolites and a mechanistic biomarker that could be pursued in future clinical studies. Furthermore, our work adds to the growing literature linking the microbiome to mitochondrial function, raising intriguing questions as to the bidirectional communication between our endo- and ecto-symbionts.\n                <\/jats:p>","DOI":"10.1101\/2025.03.24.645052","type":"posted-content","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T04:42:36Z","timestamp":1742964156000},"source":"Crossref","is-referenced-by-count":0,"title":["Gut bacterial lactate stimulates lung epithelial mitochondria and exacerbates acute lung injury"],"prefix":"10.64898","author":[{"given":"Vaibhav","family":"Upadhyay","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"},{"name":"Department of Medicine, Division of Pulmonary and Critical Care, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin F.","family":"Ortega","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"Ramirez Hernandez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gagandeep","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Trepka","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel R.","family":"Rock","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafaella T.","family":"Shima","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco; San Francisco, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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