{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:58:32Z","timestamp":1768521512327,"version":"3.49.0"},"posted":{"date-parts":[[2025,3,31]]},"group-title":"Developmental Biology","reference-count":83,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T00:00:00Z","timestamp":1743379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,31]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Somatic sex identity must be maintained throughout adulthood for tissue function. Adult somatic stem cells in the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  testis (i.e., CySCs) lacking the transcription factor Chinmo are reprogrammed to their ovarian counterparts by induction of female-specific Tra\n                  <jats:sup>F<\/jats:sup>\n                  , but this is not mechanistically understood. Pioneer factors play central roles in direct reprogramming, and many upregulated genes in\n                  <jats:italic>\n                    chinmo\n                    <jats:sup>\u2212\/-<\/jats:sup>\n                  <\/jats:italic>\n                  CySCs contain binding sites for the pioneer factor Zelda (Zld). microRNAs repress\n                  <jats:italic>zld<\/jats:italic>\n                  mRNA in wild type CySCs, but they are downregulated after Chinmo loss, allowing for\n                  <jats:italic>zld<\/jats:italic>\n                  mRNA translation. Zld depletion from\n                  <jats:italic>chinmo<\/jats:italic>\n                  <jats:sup>\u2212\/-<\/jats:sup>\n                  CySCs suppresses feminization, and ectopic Zld induces Tra\n                  <jats:sup>F<\/jats:sup>\n                  and feminizes wild-type CySCs.\n                  <jats:italic>qkr58E-2<\/jats:italic>\n                  and\n                  <jats:italic>ecdysone receptor<\/jats:italic>\n                  (\n                  <jats:italic>EcR<\/jats:italic>\n                  ), direct Zld targets in the embryo, are female-biased in adult gonads and upregulated in\n                  <jats:italic>chinmo<\/jats:italic>\n                  <jats:sup>\u2212\/-<\/jats:sup>\n                  CySCs. The RNA-binding protein Qkr58E-2 produces Tra\n                  <jats:sup>F<\/jats:sup>\n                  , while EcR promotes female-biased gene expression. Ectopic Zld feminizes adult male adipose tissue, demonstrating that Zld can instruct female and override male identity in adult XY tissues.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>\n                        <jats:italic>zld<\/jats:italic>\n                        mRNA is repressed by microRNAs in XY somatic gonadal cells\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        Zld is upregulated in and required for sex reversal of XY\n                        <jats:italic>chinmo<\/jats:italic>\n                        <jats:sup>\u2212\/-<\/jats:sup>\n                        cells\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        Zld induces Qkr58E-2 and EcR, which cause Tra\n                        <jats:sup>F<\/jats:sup>\n                        and female-biased transcription\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        Zld feminizes XY adipose cells by inducing Tra\n                        <jats:sup>F<\/jats:sup>\n                        and downregulating Chinmo\n                      <\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.1101\/2025.03.26.645575","type":"posted-content","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T23:55:21Z","timestamp":1743465321000},"source":"Crossref","is-referenced-by-count":0,"title":["The pioneer factor Zelda induces male-to-female somatic sex reversal in adult 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