{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:46:38Z","timestamp":1779324398309,"version":"3.51.4"},"posted":{"date-parts":[[2025,4,3]]},"group-title":"Genetics","reference-count":36,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,4,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  First identified in 2009,\n                  <jats:italic>Candidozyma auris<\/jats:italic>\n                  (formerly\n                  <jats:italic>Candida auris<\/jats:italic>\n                  ) is an emerging multidrug resistant fungus that can cause invasive infections with a crude mortality rate ranging from 30-60%. Currently, 30-50% of\n                  <jats:italic>C. auris<\/jats:italic>\n                  isolates are intrinsically resistant to amphotericin B. In this work, we characterized a clinical case of acquired amphotericin B resistance using whole genome sequencing, a large-scale phenotypic screen, comprehensive sterol profiling, and genotypic reversion using CRISPR. Data obtained in this work provides evidence that a deletion resulting in a frameshift in\n                  <jats:italic>ERG3<\/jats:italic>\n                  contributes to the observed resistant phenotype.\n                <\/jats:p>\n                <jats:p>\n                  Characterization of this isolate also revealed a fitness cost is associated with the abrogation of ergosterol production and its replacement with other late-stage sterols. This article presents a clinical case description of amphotericin B resistance from a frameshift mutation in\n                  <jats:italic>ERG3<\/jats:italic>\n                  in\n                  <jats:italic>C. auris<\/jats:italic>\n                  and marks an advancement in the understanding of antifungal resistance in this fungal pathogen.\n                <\/jats:p>","DOI":"10.1101\/2025.03.30.646105","type":"posted-content","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T00:25:43Z","timestamp":1743726343000},"source":"Crossref","is-referenced-by-count":4,"title":["Acquired Amphotericin B Resistance Attributed to a Mutated\n                  <i>ERG3<\/i>\n                  in\n                  <i>Candidozyma auris<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7245-3182","authenticated-orcid":false,"given":"Lauryn","family":"Massic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-9561","authenticated-orcid":false,"given":"Laura A","family":"Doorley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah J","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Denise","family":"Siao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren","family":"Siao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2158-3175","authenticated-orcid":false,"given":"Philip","family":"Dykema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Hua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5778-960X","authenticated-orcid":false,"given":"Christina A.","family":"Cuomo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. David","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Van Hooser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-4194","authenticated-orcid":false,"given":"Josie E","family":"Parker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven L","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Hess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9317-0935","authenticated-orcid":false,"given":"Jeffrey M","family":"Rybak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0334-1946","authenticated-orcid":false,"given":"Mark","family":"Pandori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025040711401031000_2025.03.30.646105v1.1","unstructured":"@CDCgov. 2019 Antibiotic Resistance Threats Report | CDC. 2022 2022-07-15T02:03:14Z [cited; Available from: https:\/\/www.cdc.gov\/drugresistance\/biggest-threats.html"},{"key":"2025040711401031000_2025.03.30.646105v1.2","doi-asserted-by":"publisher","DOI":"10.3767\/persoonia.2024.52.02"},{"key":"2025040711401031000_2025.03.30.646105v1.3","unstructured":"@CDCgov. 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