{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:23:23Z","timestamp":1785435803666,"version":"3.56.0"},"posted":{"date-parts":[[2025,4,14]]},"group-title":"Cell Biology","reference-count":92,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T00:00:00Z","timestamp":1744588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R01 AI125534"],"award-info":[{"award-number":["R01 AI125534"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2023198"],"award-info":[{"award-number":["2023198"]}],"id":[{"id":"https:\/\/ror.org\/00j8z2m73","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EPAR2216"],"award-info":[{"award-number":["EPAR2216"]}],"id":[{"id":"https:\/\/ror.org\/05gxnyn08","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Blood-stage\n                  <jats:italic>Plasmodium falciparum<\/jats:italic>\n                  parasites rely on a non-photosynthetic plastid, the apicoplast, for survival, making it an attractive target for antimalarial intervention. Like the mitochondrion, the apicoplast cannot be generated\n                  <jats:italic>de novo<\/jats:italic>\n                  and must be inherited by daughter parasites during cell division. This inheritance relies on coordinated apicoplast positioning and fission, but the molecular mechanisms controlling these processes remain poorly understood. Here, we identify a previously uncharacterized\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  protein (Pf3D7_0613600), which we name PfAnchor, as a key regulator of apicoplast fission. Using Ultrastructure Expansion Microscopy (U-ExM), we show that PfAnchor localizes to the apicoplast throughout the asexual blood-stage. Conditional depletion disrupts apicoplast fission, leading to incomplete cytokinesis and parasite death. Notably, loss of the apicoplast\u2019s elongated branched structure via azithromycin treatment rescues these defects, underscoring Anchor\u2019s specific role in apicoplast fission. Immunoprecipitation identified an interaction with the dynamin-like GTPase PfDyn2, a key mediator of both apicoplast and mitochondrial fission, establishing PfAnchor as the first apicoplast-specific dynamin adaptor protein. Our findings define PfAnchor as an essential factor for apicoplast fission and inheritance in\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  blood-stage parasites, highlighting parasite-specific organelle division as a potential vulnerability for therapeutic intervention.\n                <\/jats:p>","DOI":"10.1101\/2025.04.12.648511","type":"posted-content","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:10:14Z","timestamp":1744675814000},"source":"Crossref","is-referenced-by-count":0,"title":["An Essential Adaptor for Apicoplast Fission and Inheritance in Malaria Parasites"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7639-2800","authenticated-orcid":false,"given":"James","family":"Blauwkamp","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine, Department of Biochemistry Molecular Biology and Pharmacology, Indianapolis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4830-5471","authenticated-orcid":false,"given":"Krithika","family":"Rajaram","sequence":"additional","affiliation":[{"name":"Johns Hopkins Bloomberg School of Public Health, Department of Molecular Microbiology and Immunology, Baltimore"},{"name":"The Ohio State University College of Arts and Sciences, Department of Microbiology, Columbus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8157-6372","authenticated-orcid":false,"given":"Sophia R.","family":"Staggers","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Department of Structural Biology, Pittsburgh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6240-3518","authenticated-orcid":false,"given":"Oliver","family":"Harrigan","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Department of Biochemistry Molecular Biology and Pharmacology, Indianapolis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0049-0073","authenticated-orcid":false,"given":"Emma H.","family":"Doud","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Department of Biochemistry Molecular Biology and Pharmacology, Indianapolis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[{"name":"Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2006-3987","authenticated-orcid":false,"given":"Hangjun","family":"Ke","sequence":"additional","affiliation":[{"name":"Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College 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