{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:26:58Z","timestamp":1785403618336,"version":"3.56.0"},"posted":{"date-parts":[[2025,5,7]]},"group-title":"Animal Behavior and Cognition","reference-count":41,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R01 AA030048"],"award-info":[{"award-number":["R01 AA030048"]}],"id":[{"id":"https:\/\/ror.org\/02jzrsm59","id-type":"ROR","asserted-by":"publisher"}]},{"award":["T32 AA007456"],"award-info":[{"award-number":["T32 AA007456"]}],"id":[{"id":"https:\/\/ror.org\/02jzrsm59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Alcohol use disorder (AUD) imposes a significant global health burden, yet effective treatments remain limited due to the scarcity of well-characterized biological sample repositories. To address this gap, we established the UCSD Alcohol BioBank, a comprehensive resource containing thousands of samples from over 700 genetically diverse heterogeneous stock (HS) rats. Modeled after successful cocaine and oxycodone biobanks, this repository utilizes the chronic intermittent ethanol vapor exposure (CIE) model, paired with oral self-administration, to characterize AUD-like behaviors, including ethanol consumption, preference, motivation, and withdrawal symptoms such as allodynia and anxiety-like behavior. Longitudinal samples (blood, urine, and feces) are collected before, during, and after ethanol exposure, while terminal samples (brain, heart, liver, kidneys, cecum, reproductive organs, adrenal glands, peripheral blood mononuclear cells) are obtained at intoxication, acute withdrawal, protracted abstinence, or from naive controls. Samples are preserved via snap-freezing or paraformaldehyde fixation to support diverse applications, including genomics, transcriptomics, proteomics, and neuroanatomy. The genetic diversity of HS rats enables genome-wide association studies (GWAS) to identify AUD-related genetic variants. Freely available to non-profit organizations at\n                  <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.alcoholbiobank.org\">www.alcoholbiobank.org<\/jats:ext-link>\n                  , with genetic and behavioral data deposited in public repositories, the Alcohol BioBank facilitates collaborative research to uncover biomarkers and develop novel therapies for AUD, addressing a critical need in addiction science.\n                <\/jats:p>","DOI":"10.1101\/2025.04.30.651600","type":"posted-content","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T12:20:22Z","timestamp":1746620422000},"source":"Crossref","is-referenced-by-count":2,"title":["A Preclinical Alcohol BioBank: Samples from Behaviorally Characterized HS Rats for AUD Research"],"prefix":"10.64898","author":[{"given":"Michelle R.","family":"Doyle","sequence":"first","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Campo","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selen","family":"Dirik","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Balaguer","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelica R.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marsida","family":"Kallupi","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4782-7430","authenticated-orcid":false,"given":"Giordano","family":"de Guglielmo","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041120555420000_2025.04.30.651600v1.1","first-page":"228","article-title":"The Collaborative Study on the Genetics of Alcoholism","volume":"19","year":"1995","journal-title":"Alcohol Health Res World"},{"key":"2026041120555420000_2025.04.30.651600v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-021-03151-0"},{"key":"2026041120555420000_2025.04.30.651600v1.3","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2022.832899"},{"key":"2026041120555420000_2025.04.30.651600v1.4","doi-asserted-by":"crossref","unstructured":"Carrette LLG et al. (2021) The Cocaine and Oxycodone Biobanks, Two Repositories from Genetically Diverse and Behaviorally Characterized Rats for the Study of Addiction. eNeuro 8.","DOI":"10.1523\/ENEURO.0033-21.2021"},{"key":"2026041120555420000_2025.04.30.651600v1.5","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.jomfp_478_22"},{"key":"2026041120555420000_2025.04.30.651600v1.6","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22927"},{"key":"2026041120555420000_2025.04.30.651600v1.7","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1922-3"},{"key":"2026041120555420000_2025.04.30.651600v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2022.109309"},{"key":"2026041120555420000_2025.04.30.651600v1.9","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1395-16.2016"},{"key":"2026041120555420000_2025.04.30.651600v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09183-0"},{"key":"2026041120555420000_2025.04.30.651600v1.11","unstructured":"de Guglielmo G et al. (2024) Large-scale characterization of cocaine addiction-like behaviors reveals that escalation of intake, aversion-resistant responding, and breaking-points are highly correlated measures of the same construct. Elife 12."},{"key":"2026041120555420000_2025.04.30.651600v1.12","doi-asserted-by":"publisher","DOI":"10.1111\/gbb.12860"},{"key":"2026041120555420000_2025.04.30.651600v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2023.109770"},{"key":"2026041120555420000_2025.04.30.651600v1.14","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.858427"},{"key":"2026041120555420000_2025.04.30.651600v1.15","doi-asserted-by":"crossref","unstructured":"Gilpin NW , Richardson HN , Cole M , Koob GF (2008) Vapor inhalation of alcohol in rats. Curr Protoc Neurosci Chapter 9:Unit 9 29.","DOI":"10.1002\/0471142301.ns0929s44"},{"key":"2026041120555420000_2025.04.30.651600v1.16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1360-0443.2008.02197.x"},{"key":"2026041120555420000_2025.04.30.651600v1.17","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2022.790566"},{"key":"2026041120555420000_2025.04.30.651600v1.18","doi-asserted-by":"publisher","DOI":"10.1001\/jamapsychiatry.2021.1271"},{"key":"2026041120555420000_2025.04.30.651600v1.19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1984.tb05706.x"},{"key":"2026041120555420000_2025.04.30.651600v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2012.96"},{"key":"2026041120555420000_2025.04.30.651600v1.21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1915143117"},{"key":"2026041120555420000_2025.04.30.651600v1.22","doi-asserted-by":"publisher","DOI":"10.1111\/gbb.70018"},{"key":"2026041120555420000_2025.04.30.651600v1.23","doi-asserted-by":"crossref","unstructured":"Kononoff J , Kallupi M , Kimbrough A , Conlisk D , de Guglielmo G , George O (2018) Systemic and Intra-Habenular Activation of the Orphan G Protein-Coupled Receptor GPR139 Decreases Compulsive-Like Alcohol Drinking and Hyperalgesia in Alcohol-Dependent Rats. eNeuro 5.","DOI":"10.1523\/ENEURO.0153-18.2018"},{"key":"2026041120555420000_2025.04.30.651600v1.24","doi-asserted-by":"publisher","DOI":"10.1007\/7854_2021_288"},{"key":"2026041120555420000_2025.04.30.651600v1.25","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2009.110"},{"key":"2026041120555420000_2025.04.30.651600v1.26","doi-asserted-by":"crossref","unstructured":"Kuhn BN et al. (2025) Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats. Mol Psychiatry.","DOI":"10.1038\/s41380-025-02922-4"},{"key":"2026041120555420000_2025.04.30.651600v1.27","doi-asserted-by":"crossref","unstructured":"Kumaresan V , Lim Y , Juneja P , Tipton AE , de Guglielmo G , Carrette LLG , Kallupi M , Maturin L , Liu Y , George O , Zhang H (2023) Abstinence from Escalation of Cocaine Intake Changes the microRNA Landscape in the Cortico-Accumbal Pathway. Biomedicines 11.","DOI":"10.3390\/biomedicines11051368"},{"key":"2026041120555420000_2025.04.30.651600v1.28","doi-asserted-by":"publisher","DOI":"10.1111\/gbb.12909"},{"key":"2026041120555420000_2025.04.30.651600v1.29","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-021-01615-0"},{"key":"2026041120555420000_2025.04.30.651600v1.30","doi-asserted-by":"crossref","unstructured":"Okamoto F et al. (2024) Y and mitochondrial chromosomes in the heterogeneous stock rat population. G3 (Bethesda) 14.","DOI":"10.1093\/g3journal\/jkae213"},{"key":"2026041120555420000_2025.04.30.651600v1.31","unstructured":"Organization WH (2024) Global status report on alcohol and health and treatment of substance use disorders. In: Global Report."},{"key":"2026041120555420000_2025.04.30.651600v1.32","doi-asserted-by":"publisher","DOI":"10.1016\/S0893-133X(99)00167-0"},{"key":"2026041120555420000_2025.04.30.651600v1.33","unstructured":"SAMHSA CfBHSaQC (2023) 2023 National Survey on Drug Use and Health. In."},{"key":"2026041120555420000_2025.04.30.651600v1.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.tracli.2015.11.002"},{"key":"2026041120555420000_2025.04.30.651600v1.35","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2014.01.002"},{"key":"2026041120555420000_2025.04.30.651600v1.36","doi-asserted-by":"publisher","DOI":"10.21105\/joss.00025"},{"key":"2026041120555420000_2025.04.30.651600v1.37","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz1041"},{"key":"2026041120555420000_2025.04.30.651600v1.38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001779"},{"key":"2026041120555420000_2025.04.30.651600v1.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2013.08.006"},{"key":"2026041120555420000_2025.04.30.651600v1.40","doi-asserted-by":"crossref","unstructured":"Vu T , Godbole S , Carrette LLG , Maturin L , George O , Saba LM , Kechris K (2025) Identification of Plasma Metabolites Responding to Oxycodone Exposure in Rats. Metabolites 15.","DOI":"10.3390\/metabo15020095"},{"key":"2026041120555420000_2025.04.30.651600v1.41","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-023-01452-y"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.04.30.651600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:10:09Z","timestamp":1782277809000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.04.30.651600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,7]]},"references-count":41,"URL":"https:\/\/doi.org\/10.1101\/2025.04.30.651600","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1523\/ENEURO.0207-25.2025","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2025,5,7]]},"subtype":"preprint"}}