{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:16:39Z","timestamp":1791490599383,"version":"4.3.4"},"posted":{"date-parts":[[2025,5,2]]},"group-title":"Genetics","reference-count":72,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"Ministry of Science and Technology, Taiwan","award":["MOST 111-2311-B-005-011-MY3"],"award-info":[{"award-number":["MOST 111-2311-B-005-011-MY3"]}]},{"name":"National Chung Hsing University and Chun Shan Medical University","award":["NCHU-CSMU 433 11204"],"award-info":[{"award-number":["NCHU-CSMU 433 11204"]}]},{"DOI":"10.13039\/100000057","name":"National institute of general medical sciences","doi-asserted-by":"crossref","award":["R01GM085075"],"award-info":[{"award-number":["R01GM085075"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National institute of general medical sciences","doi-asserted-by":"crossref","award":["R35GM156624-01"],"award-info":[{"award-number":["R35GM156624-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["R03HD090422"],"award-info":[{"award-number":["R03HD090422"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Aging is associated with progressive tissue decline and shifts in stem cell clonality. The role of niche signals in driving these processes remains poorly understood. Using the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  testis, we identify a regulatory axis in which age-related decline of niche signals (BMPs) lead to upregulation of the co-repressor Hairless, which downregulates the RNA-binding protein Imp in aged germline stem cells (GSCs). Reduced Imp causes loss of Chinmo, a key factor in GSC aging and competition. Reduced Chinmo causes ectopic Perlecan secretion which accumulates in the testis lumen and causes GSC loss. Aging of the testis is reversed by increasing BMPs in the niche, or by overexpressing Imp or depleting Hairless in GSCs. Furthermore, GSC clones with reduced Imp or increased Hairless are more competitive, expelling wild-type neighbors and monopolizing the niche. Thus, BMPs regulate testicular niche aging through the Hairless\u2013Imp\u2013Chinmo axis and \u201cwinning\u201d GSCs usurp these aging mechanisms.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>Aged niche cells produce less BMPs, resulting in more Hairless (H) in aged GSCs<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        Elevated H represses\n                        <jats:italic>Imp<\/jats:italic>\n                        , resulting in less Chinmo and in ectopic ECM secretion\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Aging is prevented by higher BMP in niche cells, or by higher Imp or lower H in GSCs<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        GSCs with low\n                        <jats:italic>Imp<\/jats:italic>\n                        or high\n                        <jats:italic>H<\/jats:italic>\n                        exploit these aging mechanisms to colonize the GSC pool\n                      <\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.1101\/2025.05.01.651651","type":"posted-content","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T10:05:13Z","timestamp":1746180313000},"source":"Crossref","is-referenced-by-count":1,"title":["Age-related declines in niche self-renewal factors controls testis aging and spermatogonial stem cell competition through Hairless, Imp, and Chinmo"],"prefix":"10.64898","author":[{"given":"Yu","family":"Zheng","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Chien","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan"},{"name":"Institute of Cellular and Organismic Biology, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin-Kuan","family":"Chiang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Lun","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7892-2310","authenticated-orcid":false,"given":"Hwei-Jan","family":"Hsu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan"},{"name":"Institute of Cellular and Organismic Biology, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Sung","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5997-4489","authenticated-orcid":false,"given":"Erika A.","family":"Bach","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Pharmacology,"},{"name":"Helen L. and Martin S. 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