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Here, we present a methodological platform for assembling transmembrane glycoprotein vaccine candidates into lipid nanodiscs. We demonstrate the utility of the nanodiscs in HIV membrane proximal external region (MPER)-targeting vaccine development by binding assays using surface plasmon resonance (SPR), ex vivo B cell sorting with fluorescence-activated cell sorting (FACS), and by determining the structure of a prototypical HIV MPER-targeting immunogen nanodisc in complex with three broadly neutralizing antibodies (bnAbs), including the MPER bnAb 10E8, to 3.5 \u00c5 by cryogenic electron microscopy (cryo-EM), providing a template for structure-based immunogen design for MPER. Overall, the platform offers a tool for accelerating the development of next-generation viral vaccines.<\/jats:p>","DOI":"10.1101\/2025.05.02.651272","type":"posted-content","created":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T21:55:11Z","timestamp":1746395711000},"source":"Crossref","is-referenced-by-count":4,"title":["Virus glycoprotein nanodisc platform for vaccine design"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3158-9183","authenticated-orcid":false,"given":"Kimmo","family":"Rantalainen","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA"},{"name":"IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, USA"},{"name":"Consortium for HIV\/AIDS Vaccine Development (CHAVD), The Scripps Research Institute, La Jolla, CA, 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