{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:10:06Z","timestamp":1792786206215,"version":"4.5.0"},"posted":{"date-parts":[[2025,5,6]]},"group-title":"Developmental Biology","reference-count":63,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01-HD073499-0"],"award-info":[{"award-number":["R01-HD073499-0"]}]},{"id":[{"id":"https:\/\/ror.org\/03vek6s52","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Howard Hughes Medical Institute","id":[{"id":"https:\/\/ror.org\/006w34k90","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Google Cloud Academic Research Credits Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Orchestrated control of proliferation of multiple cell types is essential for building a healthy organ. Here we use larval ovary development in\n                  <jats:italic>Drosophila melanogaster<\/jats:italic>\n                  as a model to understand the homeostasis of somatic and germ line cells in the formation of the female adult reproductive organ. We previously showed that the highly conserved Hippo signaling pathway regulates proliferation of both germ line and somatic cells in the\n                  <jats:italic>D. melanogaster<\/jats:italic>\n                  larval ovary. Response to Hippo signaling appeared to be mediated by different genetic mechanisms in germ line and soma, but the mechanism allowing distinct responses to the same signaling pathway remained unknown. Here we perform cell type-specific RNA-Seq of isolated germ line and somatic cells from the developing ovary at multiple time points spanning the formation of germ line stem cell niches, in either a Hippo signaling loss- or gain-of-function genetic background. Applying network analysis to these data revealed a novel regulator of ovarian development, the transcription factor\n                  <jats:italic>chinmo<\/jats:italic>\n                  . Subsequent experimental validation showed that\n                  <jats:italic>chinmo<\/jats:italic>\n                  acts as a key germ cell-specific translator of Hippo signaling in the developing ovary, allowing the Hippo signal to be transduced in cell type-specific ways in germ line and somatic cells within the same organ.\n                <\/jats:p>","DOI":"10.1101\/2025.05.02.651944","type":"posted-content","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T04:40:12Z","timestamp":1746506412000},"source":"Crossref","is-referenced-by-count":0,"title":["Chinmo is a novel regulator of differential Hippo signaling response within a single developing organ"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1207-8131","authenticated-orcid":false,"given":"Shreeharsha","family":"Tarikere","sequence":"first","affiliation":[{"name":"Department of Organismic & Evolutionary Biology, Harvard University, Cambridge MA, USA"},{"name":"Department of Human Genetics, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4071-4342","authenticated-orcid":false,"given":"Tarun","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Organismic & Evolutionary Biology, Harvard University, Cambridge MA, USA"},{"name":"Howard Hughes Medical Institute, Bethesda MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4540-0131","authenticated-orcid":false,"given":"Guillem","family":"Ylla","sequence":"additional","affiliation":[{"name":"Department of Organismic & Evolutionary Biology, Harvard University, Cambridge MA, USA"},{"name":"Laboratory of Bioinformatics and Genome Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2922-5855","authenticated-orcid":false,"given":"Cassandra G.","family":"Extavour","sequence":"additional","affiliation":[{"name":"Department of Organismic & Evolutionary Biology, Harvard University, Cambridge MA, USA"},{"name":"Howard Hughes Medical Institute, Bethesda MD, USA"},{"name":"Department of Molecular & Cellular Biology, Harvard University, Cambridge MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091717051036000_2025.05.02.651944v2.1","doi-asserted-by":"publisher","DOI":"10.2144\/00294bm10"},{"key":"2026091717051036000_2025.05.02.651944v2.2","doi-asserted-by":"crossref","unstructured":"Barresi MJF , Gilbert SF . 2023. 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