{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T13:12:03Z","timestamp":1768569123067,"version":"3.49.0"},"posted":{"date-parts":[[2025,5,6]]},"group-title":"Neurology","reference-count":39,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>INTRODUCTION<\/jats:title>\n                  <jats:p>Most available p-tau217 immunoassays have similar performances. It is unclear if this is due to the use of the same antibody (the \u201cALZpath antibody\u201d). We established and evaluated a novel p-tau217 assay that employs an alternative antibody, and benchmarked the results against ALZpath-p-tau217.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>Following development and analytical validation of the University of Pittsburgh (\u201cPitt-p-tau217\u201d) method, clinical verification was performed in three independent cohorts (n=363).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>\n                    Pitt-p-tau217 demonstrated high between-run stability, linearity, and specificity. Clinically, Pitt-p-tau217 differentiated neuropathologically confirmed\n                    <jats:italic>PSEN1<\/jats:italic>\n                    mutation carriers from controls with AUC=0.94, and A\u03b2-PET-positive from A\u03b2-PET-negative cognitively normal older adults with AUC up to 0.84, equivalent to ALZpath-p-tau217 results. Both Pitt-p-tau217 and ALZpath-p-tau217 were elevated in tau-PET-positive versus tau-PET-negative participants (P=0.06; AUC=0.71 for both). Between-assay correlations were up to 0.93.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>DISCUSSION<\/jats:title>\n                  <jats:p>The new Pitt-p-tau217 assay exhibits high and reproducible classification accuracies for identifying individuals with biological evidence of AD, equivalent to the widely used ALZpath-p-tau217.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.05.03.25326526","type":"posted-content","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T15:45:20Z","timestamp":1746546320000},"source":"Crossref","is-referenced-by-count":1,"title":["Pittsburgh plasma p-tau217: classification accuracies for autosomal dominant and sporadic Alzheimer\u2019s disease in the 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