{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T20:44:10Z","timestamp":1783197850352,"version":"3.54.6"},"posted":{"date-parts":[[2025,5,8]]},"group-title":"Immunology","reference-count":99,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["K22AI146217"],"award-info":[{"award-number":["K22AI146217"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI171697"],"award-info":[{"award-number":["R21AI171697"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35GM151347"],"award-info":[{"award-number":["R35GM151347"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["Graduate Research Fellowship"],"award-info":[{"award-number":["Graduate Research Fellowship"]}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["Postgraduate Scholarship \u2013 Doctoral"],"award-info":[{"award-number":["Postgraduate Scholarship \u2013 Doctoral"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Mucosal-associated invariant T (MAIT) cells recognize microbial derivatives of riboflavin synthesis presented by the MHC class I-related (MR1) molecule. Although these metabolites are highly conserved among bacteria, the cells that present them remain unknown. Here, we show type-17 MAIT cells respond to diverse isolates of the extracellular pathogen\n                  <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                  and promote bacterial clearance. Both hematopoietic and non-hematopoietic cells mediate MR1 presentation within the lungs and mediastinal lymph nodes (meLNs). Conversely, the type-1 MAIT cell response to the intracellular pathogen\n                  <jats:italic>Francisella tularensis<\/jats:italic>\n                  requires MR1 presentation by type-2 conventional dendritic cells (cDC2s) within meLNs and ablation of these cells or their expression of MR1 renders animals more susceptible to the infection. Although MR1 is broadly expressed at homeostasis,\n                  <jats:italic>A. baumannii<\/jats:italic>\n                  enhances MR1 on macrophages and fibroblasts, while\n                  <jats:italic>F. tularensis<\/jats:italic>\n                  increases expression on cDC2s. These results demonstrate that microbial tropism dictates which APCs mediate MR1 presentation of metabolites, revealing alternative therapeutic approaches.\n                <\/jats:p>","DOI":"10.1101\/2025.05.03.652000","type":"posted-content","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:20:12Z","timestamp":1746750012000},"source":"Crossref","is-referenced-by-count":4,"title":["MAIT cell responses to intracellular and extracellular pathogens are mediated by distinct antigen presenting cells"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8833-1012","authenticated-orcid":false,"given":"Dominic","family":"Haas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5981-7766","authenticated-orcid":false,"given":"Jonathan","family":"Melamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9532-5235","authenticated-orcid":false,"given":"Gabrielle","family":"LeBlanc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhita","family":"Arun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1143-8962","authenticated-orcid":false,"given":"Andrew J.","family":"Perkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Aub\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Mack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4770-7751","authenticated-orcid":false,"given":"Michael 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