{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:42:37Z","timestamp":1776015757535,"version":"3.50.1"},"posted":{"date-parts":[[2025,5,4]]},"group-title":"Molecular Biology","reference-count":15,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T00:00:00Z","timestamp":1746316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Genome- and phenome-wide association studies implicate the RNA demethylase\n                  <jats:italic>FTO<\/jats:italic>\n                  in alcohol use disorder (AUD), yet the RNA methylation landscape in AUD remains poorly characterized. Analyzing postmortem human basolateral amygdala (BLA) tissue, a key brain region in AUD-related behaviors, we found extensive m\n                  <jats:sup>6<\/jats:sup>\n                  A hypomethylation uniquely affecting circular RNAs (circRNAs). Notably,\n                  <jats:italic>FKBP5<\/jats:italic>\n                  -hosted circRNAs (circFKBP5s) exhibited pronounced hypomethylation correlating with elevated expression of\n                  <jats:italic>FKBP5<\/jats:italic>\n                  mRNA isoforms. These findings were replicated in an animal model of alcohol dependence. Predictive analyses suggest that circFKBP5s influence genes involved in neurodevelopmental processes and neuronal identity. These findings uncover a novel aspect of AUD neurobiology linked to circRNA methylation.\n                <\/jats:p>","DOI":"10.1101\/2025.05.04.652083","type":"posted-content","created":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T23:00:10Z","timestamp":1746399610000},"source":"Crossref","is-referenced-by-count":0,"title":["CircRNA hypomethylation in the human amygdala implicates\n                  <i>FKBP5<\/i>\n                  in alcohol use disorder"],"prefix":"10.64898","author":[{"given":"Tara","family":"Ghandour","sequence":"first","affiliation":[{"name":"Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill R.","family":"Glausier","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun","family":"Asok","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Columbia University, New York, NY 10032, USA"},{"name":"Mortimer B. Zuckerman Mind Brain Behavior Institute, Jerome L. Greene Science Center, New York, NY 10027, USA"},{"name":"Alien Therapeutics Inc, Philadelphia, PA, 19128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle R.","family":"Doyle","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, La Jolla, CA, 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Campo","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, La Jolla, CA, 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Colnaghi","sequence":"additional","affiliation":[{"name":"Division of Neuroscience, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy"},{"name":"School of Medicine, Vita-Salute San Raffaele University, 20132 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise B.","family":"Kandel","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Vagelos College of Physicians and Surgeons and Mailman School of Public Health, Columbia University, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric R.","family":"Kandel","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Columbia University, New York, NY 10032, USA"},{"name":"Mortimer B. Zuckerman Mind Brain Behavior Institute, Jerome L. Greene Science Center, New York, NY 10027, USA"},{"name":"Department of Psychiatry, Vagelos College of Physicians and Surgeons and Mailman School of Public Health, Columbia University, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giordano","family":"de Guglielmo","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of California San Diego, La Jolla, CA, 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao-shan Carol","family":"Huang","sequence":"additional","affiliation":[{"name":"Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7889-9250","authenticated-orcid":false,"given":"Philippe A.","family":"Melas","sequence":"additional","affiliation":[{"name":"Center for Psychiatry Research, Department of Clinical Neuroscience, Karolinska Institutet & Stockholm Health Care Services, 11364 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041209523580000_2025.05.04.652083v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s44220-024-00353-8"},{"key":"2026041209523580000_2025.05.04.652083v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11916-0"},{"key":"2026041209523580000_2025.05.04.652083v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-68578-2"},{"key":"2026041209523580000_2025.05.04.652083v1.4","unstructured":"Q. 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