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We demonstrate that salamander influenza-like virus (SILV) HA is functionally divergent from influenza B virus HA and does not bind to \u03b1 2,3- and \u03b12,6-linked sialic acids. However, the HAs of Siamese algae-eater influenza-like virus (SAEILV) and chum salmon influenza-like virus (CSILV) bind to \u03b12,3 linked sialic acid. Furthermore, SAEILV HA binds to sialyated Lewis X, is activated by human airway enzymes and is fusogenic at a wide range of pH conditions. SAEILV NA has a highly conserved active site and a similar structure to other known NAs. We also determined the cryo-electron microscopy structure of the HA of a previously described virus from the same sister clade, the Wuhan spiny eel influenza virus (WSEIV). Importantly, no cross-reactive antibodies against these HAs or NAs were found in the human serum, suggesting that humans are immunologically na\u00efve to these viruses.<\/jats:p>\n                <jats:sec>\n                  <jats:title>One sentence summary<\/jats:title>\n                  <jats:p>Novel influenza like-viruses, displayed different target receptor specificity and limited antigenic conservation of the HA and NA relative to influenza B Viruses.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.05.08.652883","type":"posted-content","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:20:14Z","timestamp":1746750014000},"source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the glycoproteins of novel fish influenza B-like viruses"],"prefix":"10.64898","author":[{"given":"Gagandeep","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, 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