{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:49:59Z","timestamp":1782280199628,"version":"3.54.5"},"posted":{"date-parts":[[2025,5,14]]},"group-title":"Microbiology","reference-count":36,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"ACLAM Foundation"},{"name":"NIH","award":["P30 CA008748"],"award-info":[{"award-number":["P30 CA008748"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Chlamydia muridarum<\/jats:italic>\n                  (Cm) has reemerged as a prevalent infectious agent in research mouse colonies. Despite its prevalence and ability to persistently colonize the murine gastrointestinal tract, few studies have evaluated the potential impact of Cm on experimental models of gastrointestinal disease. Studies were conducted to evaluate the impact of Cm on the\n                  <jats:italic>Citrobacter rodentium<\/jats:italic>\n                  (Cr),\n                  <jats:italic>Trichuris muris<\/jats:italic>\n                  (Tm) and\n                  <jats:italic>Il<\/jats:italic>\n                  10\n                  <jats:sup>-\/-<\/jats:sup>\n                  mouse models of intestinal inflammation, as well as on tumorigenesis in the Apc\n                  <jats:sup>Min\/+<\/jats:sup>\n                  mouse following administration of DSS. Na\u00efve C57BL\/6J (B6), B6.129P2-\n                  <jats:italic>Il<\/jats:italic>\n                  10\n                  <jats:italic>\n                    <jats:sup>tm1Cgn<\/jats:sup>\n                  <\/jats:italic>\n                  \/J (\n                  <jats:italic>Il<\/jats:italic>\n                  10\n                  <jats:sup>-\/-<\/jats:sup>\n                  ) and C57BL\/6J-Apc\n                  <jats:sup>Min<\/jats:sup>\n                  \/J (Apc\n                  <jats:sup>Min\/+<\/jats:sup>\n                  ) mice were infected with Cm by cohousing with chronic Cm-shedding BALB\/cJ mice for 2 weeks; controls were cohoused with Cm-free mice. After cohousing, B6 mice (n=8 Cm-infected and free) were infected with\n                  <jats:italic>Citrobacter rodentium<\/jats:italic>\n                  (Cr; 10\n                  <jats:sup>9<\/jats:sup>\n                  CFU orally) or\n                  <jats:italic>Trichuris muris<\/jats:italic>\n                  (Tm; 200 ova orally).\n                  <jats:italic>Il1<\/jats:italic>\n                  0\n                  <jats:sup>-\/-<\/jats:sup>\n                  mice (n=8\/group with and without\n                  <jats:italic>Helicobacter hepaticus<\/jats:italic>\n                  [10\n                  <jats:sup>8<\/jats:sup>\n                  CFU\/mouse] and with and without Cm) and Apc\n                  <jats:sup>Min\/+<\/jats:sup>\n                  mice (n=8\/group) received 2% DSS for 7 days in drinking water after cohousing. Mice were sacrificed 14-days post-Cr infection, 18-days post-Tm infection, 70-days after cessation of cohousing with\n                  <jats:italic>Il<\/jats:italic>\n                  10\n                  <jats:sup>-\/-<\/jats:sup>\n                  mice, and 28-days post-DSS administration to Apc\n                  <jats:sup>Min\/+<\/jats:sup>\n                  mice. The severity of the cecal and colonic lesions were evaluated and graded using a tiered, semi-quantitative scoring system. Cm infection attenuated colitis associated with Cr (p=0.03), had no effect on Tm associated pathology (p=0.22), worsened colitis in\n                  <jats:italic>Il<\/jats:italic>\n                  10\n                  <jats:sup>-\/-<\/jats:sup>\n                  mice in the absence of\n                  <jats:italic>H. hepaticus<\/jats:italic>\n                  (p=0.007), and reduced chemically induced colonic tumorigenesis in Apc\n                  <jats:sup>Min\/+<\/jats:sup>\n                  mice (p=0.004). Thus, Cm colonization differentially impacts several models of intestinal inflammation and tumorigenesis, and the presence of this bacterium in mouse colonies should be considered as a variable in these experimental readouts.\n                <\/jats:p>","DOI":"10.1101\/2025.05.14.654029","type":"posted-content","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:45:16Z","timestamp":1747269916000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Chlamydia muridarum<\/i>\n                  Infection Impacts Murine Models of Intestinal Inflammation and Cancer"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5371-3491","authenticated-orcid":false,"given":"Glory","family":"Leung","sequence":"first","affiliation":[{"name":"Tri-Institutional Training Program in Laboratory Animal Medicine and Science, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian E.","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Tri-Institutional Training Program in Laboratory Animal Medicine and Science, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, NY"},{"name":"Center of Comparative Medicine and Pathology, Memorial Sloan Kettering Cancer Center and Weill Cornell Medicine, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J","family":"Mourino","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Bar Harbor, ME"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mert","family":"Aydin","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Bar Harbor, ME"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Mammone","sequence":"additional","affiliation":[{"name":"Center of Comparative Medicine and Pathology, Memorial Sloan Kettering Cancer Center and Weill Cornell Medicine, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory F.","family":"Sonnenberg","sequence":"additional","affiliation":[{"name":"Joan and Sanford I. Weill Department of Medicine, Division of Gastroenterology & Hepatology, Weill Cornell Medicine, Cornell University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gretchen","family":"Diehl","sequence":"additional","affiliation":[{"name":"Memorial Sloan Kettering Cancer Center, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Artis","sequence":"additional","affiliation":[{"name":"Joan and Sanford I. Weill Department of Medicine, Department of Microbiology and Immunology, Weill Cornell Medicine, Cornell University, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yano","sequence":"additional","affiliation":[{"name":"Joan and Sanford I. 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