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Religious Orders Study (ROS), Rush Memory and Aging Project (MAP) and Minority Aging Research Study (MARS), that began enrollment in 1994, 1997, and 2004, respectively.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Setting<\/jats:title>\n                  <jats:p>Older priests, nuns, and brothers from across the U.S. (ROS), older adults (MAP) and older African-American adults (MARS) from across the greater Chicago metropolitan area.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Participants<\/jats:title>\n                  <jats:p>1,028 decedents with multi-omic data from the dorsolateral prefrontal cortex (DLPFC), the anterior cingulate cortex (AC), and the posterior cingulate gyrus (PCG).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Exposure(s)<\/jats:title>\n                  <jats:p>Eight layers of omics (DNA methylation and histone acetylation from DLPFC; RNA seq from AC, DLPFC, and PCG, single-nucleus RNA, TMT proteomics and metabolomics from DLPFC) and Neuroticism using the 12-item version from the NEO Five-Factor Inventory.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Main outcome(s) and measure(s)<\/jats:title>\n                  <jats:p>Person-specific multi-omic molecular pseudotime representing molecular progression from low to high phenotypic expression of neuroticism, and three multi-omic brain molecular subtypes of neuroticism which represent distinct omic pathways from no\/low neuroticism to high neuroticism that differ by their omic constituents.<\/jats:p>\n                  <jats:p>Participants are exclusively assigned to the subtype which aligns mostly with their multi-omic profile.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    The top drivers of subtype differentiation were transcriptomic alterations across three brain regions (DLPFC, AC, PCG) which extensively and differentially characterized the subtypes. The subtypes were also differentially associated with AD pathology, temporal lobe atrophy, and AD dementia, with subtype N\n                    <jats:sub>1<\/jats:sub>\n                    showing the strongest associations.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions and Relevance<\/jats:title>\n                  <jats:p>\n                    Neuroticism may be driven by three distinct molecular subtypes, with subtype N\n                    <jats:sub>1<\/jats:sub>\n                    driving ADRD-related associations, N\n                    <jats:sub>2<\/jats:sub>\n                    showing some ADRD associations, and N\n                    <jats:sub>3<\/jats:sub>\n                    being completely independent of these outcomes. Our data provide novel insights into the biology of individual differences in predispositions of neuroticism and its associations with ADRD traits.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Key points<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Question<\/jats:title>\n                    <jats:p>What are the brain multi-omics molecular signatures linking neuroticism with Alzheimer\u2019s diseases and related dementias (AD\/ADRDs)?<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>\n                      We identified three distinct brain multi-omic molecular subtypes reflecting different molecular pathways underlying neuroticism. Top omic features of the subtypes were extensively and differentially characterized by transcriptomic alterations across three brain regions \u2013 dorsolateral prefrontal cortex, anterior cingulate cortex, and posterior cingulate gyrus. Subtype N\n                      <jats:sub>1<\/jats:sub>\n                      was strongly associated with AD pathology, AD dementia, and temporal lobe atrophy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Meaning<\/jats:title>\n                    <jats:p>The association we typically observe between phenotypic neuroticism and ADRD clinical traits might be largely driven by a molecular pathway underlying this trait.<\/jats:p>\n                  <\/jats:sec>\n                <\/jats:sec>","DOI":"10.1101\/2025.05.19.654904","type":"posted-content","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T19:45:13Z","timestamp":1747856713000},"source":"Crossref","is-referenced-by-count":0,"title":["Brain Multi-Omic Subtypes of Neuroticism reveal molecular signatures linked to Alzheimer\u2019s 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