{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T06:07:18Z","timestamp":1770358038639,"version":"3.49.0"},"posted":{"date-parts":[[2025,5,25]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":30,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T00:00:00Z","timestamp":1748131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,5,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Dengue virus is a mosquito transmitted flavivirus that cause inapparent or mild disease but can also cause severe hemorrhagic fever in humans. Cross-reactive IgG antibodies can cause under specific conditions antibody dependent enhancement (ADE) of the infection via Fc-receptor interaction. Antibodies from SARS-CoV-2 (anti-S) causing COVID-19 disease can also cross-react against the dengue envelope (anti-E). We therefore investigated the antibody profile of these cross-reactive antibodies and their ability to induce ADE. We found that the cross-reactive anti-E in COVID-19 vaccinated are dominated by IgM\/A while, anti-S from vaccinated or anti-E from dengue infected patients have an IgG1 dominated antibody profile. The low-level anti-E IgG from COVID-19 vaccinated are dominated by low Fc-affinity IgG2\/4 subclass. These antibodies were able to induce in vitro ADE stronger than from Dengue infected for the 1\n                  <jats:sup>st<\/jats:sup>\n                  ,2\n                  <jats:sup>nd<\/jats:sup>\n                  dose of the vaccine or later omicron variant booster. This could partially be explained that ADE was inhibited by the complement system in dengue infected but not in COVID-19 vaccinated.\n                <\/jats:p>","DOI":"10.1101\/2025.05.23.25328123","type":"posted-content","created":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T13:35:12Z","timestamp":1748180112000},"source":"Crossref","is-referenced-by-count":2,"title":["COVID-19 Vaccination Induces Cross-Reactive Dengue Antibodies with Altered Isotype Profiles and In Vitro ADE"],"prefix":"10.64898","author":[{"given":"Sebastian","family":"Reinig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chin","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng-Yu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuei-Ching","family":"Hsiung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Kai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etsuro","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ing-Kit","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Yen","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Min","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4874-401X","authenticated-orcid":false,"given":"Shin-Ru","family":"Shih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025052708250702000_2025.05.23.25328123v1.1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2020-0572"},{"key":"2025052708250702000_2025.05.23.25328123v1.2","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PPAT.1011223"},{"key":"2025052708250702000_2025.05.23.25328123v1.3","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.975398"},{"key":"2025052708250702000_2025.05.23.25328123v1.4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.941923"},{"key":"2025052708250702000_2025.05.23.25328123v1.5","doi-asserted-by":"crossref","unstructured":"Sarker S , Dutta C , Mallick A , et al. 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