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Recent work has identified changes in corticotropin releasing factor signaling in limbic system structures, such as the amygdala and hypothalamus, as a key mechanism behind these changes \u2013 albeit in a sex-dependent manner. CRF has two major receptors, CRHR1 and CRHR2 which have also been shown to play key roles in fear and pain expression. The current work examines the effects of early life pain designed to mimic the neonatal medical trauma that occurs in the Neonatal Intensive Care Unit (NICU), paired with a juvenile trauma in the form of fear conditioning, on expression of\n                  <jats:italic>crhr1<\/jats:italic>\n                  and\n                  <jats:italic>crhr2<\/jats:italic>\n                  mRNA in the central (CeA) and basolateral (BLA) amygdala as well as the paraventricular (PVN) and ventromedial (VMH) hypothalamus. While prior work has demonstrated that early life pain significantly impacts expression of the CRF ligand, the current data demonstrate that early life pain and fear conditioning largely fail to affect CRH receptor expression in the amygdala. Modest changes in expression to\n                  <jats:italic>crhr2<\/jats:italic>\n                  in a sex and region-specific manner were observed in the hypothalamus.\n                <\/jats:p>","DOI":"10.1101\/2025.05.29.656935","type":"posted-content","created":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T04:10:12Z","timestamp":1748664612000},"source":"Crossref","is-referenced-by-count":0,"title":["The Effects of Early Life Pain and Juvenile Fear Conditioning on CRF-Receptor Expression in the Amygdala and Hypothalamus"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2308-8570","authenticated-orcid":false,"given":"Michael A.","family":"Burman","sequence":"first","affiliation":[{"name":"School of Social and Behavioral Sciences Center for Excellence in the Neuroscience University of New England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared T.","family":"Zuke","sequence":"additional","affiliation":[{"name":"School of Social and Behavioral Sciences Center for Excellence in the Neuroscience University of New England"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041200224150000_2025.05.29.656935v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2017.03.003"},{"key":"2026041200224150000_2025.05.29.656935v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2006.02.007"},{"key":"2026041200224150000_2025.05.29.656935v1.3","doi-asserted-by":"publisher","DOI":"10.1542\/peds.114.1.e77"},{"key":"2026041200224150000_2025.05.29.656935v1.4","doi-asserted-by":"publisher","DOI":"10.1097\/00004703-199908000-00008"},{"key":"2026041200224150000_2025.05.29.656935v1.5","doi-asserted-by":"publisher","DOI":"10.1017\/S0012162206001010"},{"key":"2026041200224150000_2025.05.29.656935v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2014.09.018"},{"key":"2026041200224150000_2025.05.29.656935v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2013.04.009"},{"key":"2026041200224150000_2025.05.29.656935v1.8","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2013.00013"},{"key":"2026041200224150000_2025.05.29.656935v1.9","doi-asserted-by":"publisher","DOI":"10.1002\/mrdd.10074"},{"key":"2026041200224150000_2025.05.29.656935v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/J.PSYNEUEN.2012.03.010"},{"key":"2026041200224150000_2025.05.29.656935v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cophys.2019.04.011"},{"key":"2026041200224150000_2025.05.29.656935v1.12","unstructured":"Driscoll AK , Ely DM. 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