{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:14:38Z","timestamp":1785420878724,"version":"3.56.0"},"posted":{"date-parts":[[2025,6,5]]},"group-title":"Immunology","reference-count":36,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["R01-DK-063158"],"award-info":[{"award-number":["R01-DK-063158"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["F30DK132903"],"award-info":[{"award-number":["F30DK132903"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Stanford University Medical Scientist Training Program","award":["T32GM007365"],"award-info":[{"award-number":["T32GM007365"]}]},{"name":"Stanford University Medical Scientist Training Program","award":["T32GM145402"],"award-info":[{"award-number":["T32GM145402"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  In celiac disease (CeD), a gluten-dependent autoimmune disorder, transglutaminase 2 (TG2) deamidates selected glutamine residues in gluten peptides, while HLA-DQ2 presents deamidated antigens to inflammatory T cells. The cellular sources of pathogenic TG2 and DQ2 are unclear. Using chemical biology tools, we show that intestinal CD103\n                  <jats:sup>+<\/jats:sup>\n                  dendritic cells (DCs) couple cell-surface TG2 to the endocytic LRP1 receptor to simultaneously deamidate gluten antigens and concentrate them in lysosomes. In DQ2-transgenic mice, CD103\n                  <jats:sup>+<\/jats:sup>\n                  DCs loaded with deamidated antigens migrate from intestinal lamina propria and Peyer\u2019s patches into mesenteric lymph nodes, where they engage T cells. In turn, gluten antigen presentation upregulates intestinal TG2 activity. The tool (HB-230) used to establish a role of CD103\n                  <jats:sup>+<\/jats:sup>\n                  DCs in gluten antigen presentation and TG2 activation in mice also revealed that the TG2\/LRP1 pathway is active in human CD14\n                  <jats:sup>+<\/jats:sup>\n                  monocytes. Within this population of circulating monocytes, a DC subset with the gut-homing \u03b27-integrin marker is elevated in CeD patients with active disease compared to non-celiac controls or patients on a gluten-free diet. Our findings not only inform the cellular basis for gluten toxicity in CeD but they also highlight the immunologic role of an enigmatic protein of growing therapeutic relevance in CeD and other immune disorders.\n                <\/jats:p>","DOI":"10.1101\/2025.06.02.657527","type":"posted-content","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T09:05:13Z","timestamp":1749114313000},"source":"Crossref","is-referenced-by-count":0,"title":["A dendritic cell population responsible for transglutaminase 2-mediated gluten antigen presentation in celiac disease"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8579-3235","authenticated-orcid":false,"given":"Fu-Chen","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harrison A.","family":"Besser","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"},{"name":"Stanford Medical Scientist Training Program, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Rin","family":"Chun","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Albertelli","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nielsen Q.","family":"Fernandez-Becker","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, California, 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