{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:32:27Z","timestamp":1785407547402,"version":"3.56.0"},"posted":{"date-parts":[[2025,6,6]]},"group-title":"Neuroscience","reference-count":21,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R00AA027835"],"award-info":[{"award-number":["R00AA027835"]}],"id":[{"id":"https:\/\/ror.org\/02jzrsm59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Nest building is a natural behavior that can readily be analyzed in mice in the home cage environment. Nest building is involved in thermoregulation, positive motivational states, and motor function, and alterations in this behavior have been proposed as an index for ethanol withdrawal severity in mice. However, nest building outcomes after voluntary ethanol consumption have not been examined. Here, we tested male and female C57BL\/6J mice on a 4-day drinking in the dark (DID) paradigm of binge-like drinking with either ethanol or a water control and analyzed nest scores at two timepoints (48 hours and 7 days) after the last DID session. At 48 hours after the last DID session, there were no differences between the two groups in nest quality. At 7 days after DID, ethanol-drinking animals showed significantly lower nest scores than the water group (z = -2.369,\n                  <jats:italic>p<\/jats:italic>\n                  =0.030). No differences were found between the ethanol- and water-drinking groups in locomotor activity or anxiety-like behavior at this timepoint in an open field test, indicating that nest building deficits in the ethanol group were likely not due to underlying differences in these behaviors. Together, these results validate the use of nest building as a naturalistic assessment of post-ethanol behavioral changes following voluntary binge-like drinking.\n                <\/jats:p>","DOI":"10.1101\/2025.06.03.657694","type":"posted-content","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T21:15:29Z","timestamp":1749244529000},"source":"Crossref","is-referenced-by-count":0,"title":["Effects of binge-like ethanol drinking on nest building behavior in mice"],"prefix":"10.64898","author":[{"given":"Andr\u00e9 Lucas","family":"Silva-Borges","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3722-7578","authenticated-orcid":false,"given":"Amanda M.","family":"Barkley-Levenson","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"#cr-split#-2026041201245560000_2025.06.03.657694v2.1.1","unstructured":"SAMHSA. Center for Behavioral Health Statistics and Quality. 2023 National Survey on Drug Use and Health. Table 2.9B-Alcohol, binge alcohol, and heavy alcohol use in past month: among people aged 12 or older"},{"key":"#cr-split#-2026041201245560000_2025.06.03.657694v2.1.2","unstructured":"by detailed age category, percentages, 2022 and 2023. [cited 2025 May 8]. Available from: https:\/\/www.samhsa.gov\/data\/report\/2023-nsduh-detailed-tables"},{"key":"2026041201245560000_2025.06.03.657694v2.2","unstructured":"NIAAA. National Institute on Alcohol Abuse and Alcoholism. Understanding Alcohol Drinking Patterns. [cited 2025 May 8]. Available from: https:\/\/www.niaaa.nih.gov\/alcohols-effects-health\/alcohol-drinking-patterns"},{"issue":"1","key":"2026041201245560000_2025.06.03.657694v2.3","first-page":"5","article-title":"Introduction to alcohol withdrawal","volume":"22","year":"1998","journal-title":"Alcohol health and research world"},{"key":"2026041201245560000_2025.06.03.657694v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2016.08.006"},{"key":"2026041201245560000_2025.06.03.657694v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2014.02.001"},{"key":"2026041201245560000_2025.06.03.657694v2.6","doi-asserted-by":"publisher","DOI":"10.3791\/2607"},{"key":"2026041201245560000_2025.06.03.657694v2.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-3472(69)80020-5"},{"key":"2026041201245560000_2025.06.03.657694v2.8","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8728415"},{"issue":"6","key":"2026041201245560000_2025.06.03.657694v2.9","first-page":"25","article-title":"Home improvement: C57BL\/6J mice given more naturalistic nesting materials build better nests","volume":"47","year":"2008","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"2026041201245560000_2025.06.03.657694v2.10","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5412.330"},{"key":"2026041201245560000_2025.06.03.657694v2.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2016.01.023"},{"key":"2026041201245560000_2025.06.03.657694v2.12","doi-asserted-by":"publisher","DOI":"10.1186\/s13293-023-00565-0"},{"key":"2026041201245560000_2025.06.03.657694v2.13","doi-asserted-by":"publisher","DOI":"10.1002\/dev.20320"},{"key":"2026041201245560000_2025.06.03.657694v2.14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2010.01384.x"},{"key":"2026041201245560000_2025.06.03.657694v2.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2004.10.007"},{"issue":"2","key":"2026041201245560000_2025.06.03.657694v2.16","first-page":"105","article-title":"Animal models of alcohol withdrawal","volume":"24","year":"2000","journal-title":"Alcohol research & health: the journal of the National Institute on Alcohol Abuse and Alcoholism"},{"key":"2026041201245560000_2025.06.03.657694v2.17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1369-1600.2009.00194.x"},{"key":"2026041201245560000_2025.06.03.657694v2.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2022.105967"},{"key":"2026041201245560000_2025.06.03.657694v2.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2012.12.018"},{"key":"2026041201245560000_2025.06.03.657694v2.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2005.05.009"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.06.03.657694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:58:56Z","timestamp":1782277136000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.06.03.657694"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,6]]},"references-count":21,"URL":"https:\/\/doi.org\/10.1101\/2025.06.03.657694","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1038\/s41598-025-24138-w","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2025,6,6]]},"subtype":"preprint"}}