{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T01:19:47Z","timestamp":1776475187491,"version":"3.51.2"},"posted":{"date-parts":[[2025,6,4]]},"group-title":"Microbiology","reference-count":30,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The gut microbiome of eusocial corbiculate bees, which include honeybees, bumblebees, and stingless bees, consists of anciently associated, host-specific bacteria that are vital for bee health. Two symbionts,\n                  <jats:italic>Snodgrassella<\/jats:italic>\n                  and\n                  <jats:italic>Gilliamella<\/jats:italic>\n                  , are ubiquitous in honeybees and bumblebees. However, their presence varies in the stingless bee clade (Meliponini), a group with pantropical distribution. They are absent or rare in the diverse genus\n                  <jats:italic>Melipona<\/jats:italic>\n                  , indicating a shift in microbiota composition in this lineage. To identify the main members of the\n                  <jats:italic>Melipona<\/jats:italic>\n                  microbiota, we combined newly collected and published data from field-collected individuals of several species. Additionally, we identified the localization of the dominant microbiota members within the gut regions of\n                  <jats:italic>Melipona quadrifasciata anthidioides<\/jats:italic>\n                  . The dominant microbiota of\n                  <jats:italic>Melipona<\/jats:italic>\n                  species includes members of the genera\n                  <jats:italic>Bifidobacterium, Lactobacillus, Apilactobacillus, Floricoccus<\/jats:italic>\n                  , and\n                  <jats:italic>Bombella<\/jats:italic>\n                  . Among these,\n                  <jats:italic>Apilactobacillus<\/jats:italic>\n                  and\n                  <jats:italic>Bombella<\/jats:italic>\n                  dominate in the crop, whereas\n                  <jats:italic>Apilactobacillus<\/jats:italic>\n                  and other members of the Lactobacillaceae dominate the ventriculus. The ileum lacks\n                  <jats:italic>Snodgrassella<\/jats:italic>\n                  or\n                  <jats:italic>Gilliamella<\/jats:italic>\n                  but contains a putative new symbiont close to\n                  <jats:italic>Floricoccus<\/jats:italic>\n                  , as well as strains of\n                  <jats:italic>Bifidobacterium<\/jats:italic>\n                  , Lactobacillaceae (including\n                  <jats:italic>Apilactobacillus<\/jats:italic>\n                  ), and\n                  <jats:italic>Bombella<\/jats:italic>\n                  . The rectum is dominated by\n                  <jats:italic>Bifidobacterium<\/jats:italic>\n                  and\n                  <jats:italic>Lactobacillus<\/jats:italic>\n                  . In summary, the\n                  <jats:italic>Melipona<\/jats:italic>\n                  microbiota is compositionally distinct but shows spatial organization paralleling that of other eusocial corbiculate bees.\n                <\/jats:p>","DOI":"10.1101\/2025.06.03.657762","type":"posted-content","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:50:11Z","timestamp":1749084611000},"source":"Crossref","is-referenced-by-count":2,"title":["Gut microbiota of Brazilian\n                  <i>Melipona<\/i>\n                  stingless bees: dominant members and their localization in different gut regions"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9604-6243","authenticated-orcid":false,"given":"Amanda","family":"Tristao Santini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan Emanuel Silva","family":"Cerqueira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2983-9769","authenticated-orcid":false,"given":"Nancy A.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helder Canto","family":"Resende","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weyder Cristiano","family":"Santana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio Oliveira","family":"de Paula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia Canedo","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025060621000806000_2025.06.03.657762v1.1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010814-020822"},{"key":"2025060621000806000_2025.06.03.657762v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2017.03.040"},{"key":"2025060621000806000_2025.06.03.657762v1.3","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.160992"},{"key":"2025060621000806000_2025.06.03.657762v1.4","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13579"},{"key":"2025060621000806000_2025.06.03.657762v1.5","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01861-14"},{"key":"2025060621000806000_2025.06.03.657762v1.6","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiae063"},{"key":"2025060621000806000_2025.06.03.657762v1.7","doi-asserted-by":"crossref","unstructured":"Michener CD . 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Meliponini Lepeletier, 1836. de Moelo GAR , Moure JS , Urban D , da Maia E , Dal Molin A , Gon\u00e7alves RB , editors. Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region - online version. 2012. 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