{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T10:54:41Z","timestamp":1775991281451,"version":"3.50.1"},"posted":{"date-parts":[[2025,6,11]]},"group-title":"Bioengineering","reference-count":33,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"Juvenile Diabetes Research Foundation","award":["2-SRA-2022-1218-S-B"],"award-info":[{"award-number":["2-SRA-2022-1218-S-B"]}]},{"name":"National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health","award":["R03 DK135460"],"award-info":[{"award-number":["R03 DK135460"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Allogeneic cell transplantation such as beta-cell replacement for treatment of type 1 diabetes (T1D) is constrained by poor graft survival and functionality, immune rejection, and the lack of scalable biomanufacturing processes. Here, we engineered functional human islet constructs that replicate the physiomimetic human pancreatic microenvironment by employing a clinically-scalable 3D bioprinting system. To support human islet viability and function, we developed alginate-based bioinks incorporating human pancreatic decellularized extracellular matrix (dECM). These bioink formulations were optimized for shear-thinning properties for extrusion of human islets, as well as selective permeability that supports nutrient and therapeutic molecule exchange. Extrusion-based printing parameters were refined to minimize shear stress-induced damage to human islets. The resulting bioprinted pancreatic constructs demonstrated robust structural integrity, high human islet viability (&gt;85%), and long-term glucose-stimulated insulin secretion (GSIS) over a 21-days\n                  <jats:italic>in vitro<\/jats:italic>\n                  culture period, even at a high islet packing density (10,000 islet equivalent\/mL) while free islet controls displayed a significant functional decline. The higher performance of bioprinted islets maybe attributable to the supportive 3D dECM-rich microenvironment mitigating culture-induced stress by recapitulating the islet pancreatic niche. This scalable 3D dECM-alginate bioprinted platform represents a new advanced functional material for advancing clinically translatable bio-artificial pancreas therapies for T1D.\n                <\/jats:p>","DOI":"10.1101\/2025.06.06.658360","type":"posted-content","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T12:35:13Z","timestamp":1749645313000},"source":"Crossref","is-referenced-by-count":0,"title":["Scalable 3D Bioprinting of Human Islets in a Pancreatic Decellularized Extracellular Matrix-Enriched Bioink for Beta-Cell Replacement Therapy"],"prefix":"10.64898","author":[{"given":"Wonwoo","family":"Jeong","sequence":"first","affiliation":[{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quentin","family":"Perrier","sequence":"additional","affiliation":[{"name":"Departement of Surgery, Section of Transplantation, Wake Forest School of Medicine, Winston Salem, NC, USA"},{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"},{"name":"Univ. Grenoble Alpes, INSERM U1055, Department of Pharmacy, Grenoble Alpes University Hospital, LBFA, Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arunkumar","family":"Rengaraj","sequence":"additional","affiliation":[{"name":"Departement of Surgery, Section of Transplantation, Wake Forest School of Medicine, Winston Salem, NC, USA"},{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori","family":"Byers","sequence":"additional","affiliation":[{"name":"Departement of Surgery, Section of Transplantation, Wake Forest School of Medicine, Winston Salem, NC, USA"},{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grisell C.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Peveri","sequence":"additional","affiliation":[{"name":"Wake Forest University, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jake","family":"Miller","sequence":"additional","affiliation":[{"name":"Wake Forest University, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Bottino","sequence":"additional","affiliation":[{"name":"Imagine Islet Center, Imagine Pharma, Pittsburgh, PA, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexei V.","family":"Mikhailov","sequence":"additional","affiliation":[{"name":"Department of Pathology, Atrium Health Wake Forest School of Medicine, Winston-Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Fraker","sequence":"additional","affiliation":[{"name":"Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel C.","family":"Opara","sequence":"additional","affiliation":[{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice A.","family":"Tomei","sequence":"additional","affiliation":[{"name":"Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Orlando","sequence":"additional","affiliation":[{"name":"Departement of Surgery, Section of Transplantation, Wake Forest School of Medicine, Winston Salem, NC, USA"},{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amish","family":"Asthana","sequence":"additional","affiliation":[{"name":"Departement of Surgery, Section of Transplantation, Wake Forest School of Medicine, Winston Salem, NC, USA"},{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041203082719000_2025.06.06.658360v1.1","doi-asserted-by":"publisher","DOI":"10.1093\/stcltm\/szae049"},{"key":"2026041203082719000_2025.06.06.658360v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.06.027"},{"key":"2026041203082719000_2025.06.06.658360v1.3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25073778"},{"key":"2026041203082719000_2025.06.06.658360v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(18)30078-0"},{"key":"2026041203082719000_2025.06.06.658360v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(22)00114-0"},{"key":"2026041203082719000_2025.06.06.658360v1.6","doi-asserted-by":"publisher","DOI":"10.2337\/dc21-2688"},{"key":"2026041203082719000_2025.06.06.658360v1.7","doi-asserted-by":"crossref","unstructured":"Perrier Q , Jambon-Barbara C , Kessler L , Villard O , Buron F , Guerci B , et al. 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