{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:47:25Z","timestamp":1782280045720,"version":"3.54.5"},"posted":{"date-parts":[[2025,6,11]]},"group-title":"Neuroscience","reference-count":63,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"crossref","award":["K99EB028828"],"award-info":[{"award-number":["K99EB028828"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Purpose<\/jats:title>\n                  <jats:p>\n                    Spectral editing is the most common MRS approach for noninvasive in vivo measurement of low-concentration, strongly overlapped metabolites in the brain, such as \u03b3-aminobutyric acid (GABA) and glutathione (GSH). Multi-metabolite editing methods, including HERMES and HERCULES, have recently been introduced, where multiple\n                    <jats:italic>J<\/jats:italic>\n                    -coupled metabolites can be edited in a single acquisition without increasing total scan time. Yet little is known regarding the reliability of these methods. This study assessed the test-retest reliability of HERMES and HERCULES, where volume localization was achieved using either PRESS or sLASER.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Sixteen healthy adult volunteers were scanned twice in two separate sessions. Single-voxel edited MRS data were acquired in the medial parietal lobe using the following sequences: (1) HERMES-PRESS; (2) HERMES-sLASER; (3) HERCULES-PRESS; (4) HERCULES-sLASER. Spectra were processed and metabolites were quantified using the Osprey software. Data quality metrics and reliability statistics were estimated for all four acquisitions.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    HERMES-sLASER demonstrated lower within-subjects coefficients of variation (CV\n                    <jats:sub>ws<\/jats:sub>\n                    ) for GSH, glutamine (Gln), and glutamate (Glu) + Gln (Glx), suggesting improved reliability compared to HERMES-PRESS. However, GABA + co-edited macromolecules (GABA+) and Glu showed higher CV\n                    <jats:sub>ws<\/jats:sub>\n                    for HERMES-sLASER. HERCULES-sLASER produced better reliability than HERCULES-PRESS for GABA+, GSH, Glu, Gln, Glx, aspartate (Asp), and lactate (Lac).\n                    <jats:italic>N<\/jats:italic>\n                    -acetylaspartate (NAA) and\n                    <jats:italic>N<\/jats:italic>\n                    -acetylaspartylglutamate (NAAG) showed higher CV\n                    <jats:sub>ws<\/jats:sub>\n                    for HERCULES-sLASER. These findings suggest that sLASER may be more advantageous than PRESS for volume localization in simultaneous multi-metabolite editing.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Using sLASER yielded better test-retest reliability for most metabolites than using PRESS for volume localization for HERMES and HERCULES.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.06.07.657685","type":"posted-content","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T02:05:23Z","timestamp":1749693923000},"source":"Crossref","is-referenced-by-count":1,"title":["Test-retest reliability of multi-metabolite edited MRS at 3T using PRESS and sLASER"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6651-183X","authenticated-orcid":false,"given":"Jessica","family":"Archibald","sequence":"first","affiliation":[{"name":"Department of Radiology, Weill Cornell Medicine, New York, NY, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7423-895X","authenticated-orcid":false,"given":"Amy E.","family":"Bouchard","sequence":"additional","affiliation":[{"name":"Department of Radiology, Weill Cornell Medicine, New York, NY, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6193-366X","authenticated-orcid":false,"given":"Ralph","family":"Noeske","sequence":"additional","affiliation":[{"name":"GE HealthCare, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9452-2245","authenticated-orcid":false,"given":"Dikoma C.","family":"Shungu","sequence":"additional","affiliation":[{"name":"Department of Radiology, Weill Cornell Medicine, New York, NY, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0349-3782","authenticated-orcid":false,"given":"Mark","family":"Mikkelsen","sequence":"additional","affiliation":[{"name":"Department of Radiology, Weill Cornell Medicine, New York, NY, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041203115807000_2025.06.07.657685v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2017.04.030"},{"key":"2026041203115807000_2025.06.07.657685v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/s10334-023-01118-7"},{"key":"2026041203115807000_2025.06.07.657685v1.3","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.71016"},{"key":"2026041203115807000_2025.06.07.657685v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2020.116794"},{"key":"2026041203115807000_2025.06.07.657685v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-76263-3"},{"key":"2026041203115807000_2025.06.07.657685v1.6","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2027-14.2014"},{"key":"2026041203115807000_2025.06.07.657685v1.7","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2016.00014"},{"key":"2026041203115807000_2025.06.07.657685v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2017.08.022"},{"key":"2026041203115807000_2025.06.07.657685v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17741-8"},{"key":"2026041203115807000_2025.06.07.657685v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.schres.2017.07.021"},{"key":"2026041203115807000_2025.06.07.657685v1.11","doi-asserted-by":"publisher","DOI":"10.1001\/jamapsychiatry.2022.3384"},{"key":"2026041203115807000_2025.06.07.657685v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2021.12.010"},{"key":"2026041203115807000_2025.06.07.657685v1.13","doi-asserted-by":"crossref","unstructured":"Graaf RAd. 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