{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:51:50Z","timestamp":1785433910150,"version":"3.56.0"},"posted":{"date-parts":[[2025,6,11]]},"group-title":"Immunology","reference-count":144,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"id":[{"id":"https:\/\/ror.org\/027vj4x92","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Abata Therapeutics"},{"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Chronic neuroinflammation and neurodegeneration are critical but unresolved drivers of disability accumulation in progressive multiple sclerosis (MS). Chronic active white matter lesions (CAL), identifiable radiologically as paramagnetic rim lesions (PRL), indicate progression-relevant chronic neuroinflammation. Using single-cell transcriptomics (scRNAseq) and T-cell receptor sequencing (scTCR-seq), we profiled cerebrospinal fluid (CSF) and blood immune cells of 34 radiologically characterized adults with MS (17 untreated, 6 treated with B-cell-depletion) and 5 healthy controls. Coupled with proteomics, we found PRL-associated enrichment of interferon (IFN) signaling and upregulation of TCR signaling in CSF and blood. This was accompanied by clonal expansion of CD8\n                  <jats:sup>+<\/jats:sup>\n                  T effector memory (TEM) cells, with the highly expanded clonal cells exhibiting T helper type 1 (T\n                  <jats:sub>H<\/jats:sub>\n                  1) and cytotoxic profiles. Validating the cytotoxic immune profile in blood using flow cytometry, we identified a cellular correlate of PRL exhibiting features of CD8\n                  <jats:sup>+<\/jats:sup>\n                  T\n                  <jats:sub>EMRA<\/jats:sub>\n                  cells. Despite B-cell depletion, PRL-associated neuroinflammation, driven by myeloid activation and CD8\n                  <jats:sup>+<\/jats:sup>\n                  T-cell cytotoxicity, persisted. Serum and CSF proteomic networks showed PRL-pertinent signatures, including networks unaffected by B-cell depletion. Using\n                  <jats:italic>in silico<\/jats:italic>\n                  perturbation, we nominated therapeutic targets, including\n                  <jats:italic>MYD88<\/jats:italic>\n                  ,\n                  <jats:italic>TNF<\/jats:italic>\n                  ,\n                  <jats:italic>MYC<\/jats:italic>\n                  ,\n                  <jats:italic>TYK2<\/jats:italic>\n                  ,\n                  <jats:italic>JAK2,<\/jats:italic>\n                  and\n                  <jats:italic>BTK<\/jats:italic>\n                  , for alleviating chronic neuroinflammation in MS. Our findings highlight mechanisms of chronic neuroinflammation in MS and point to potential biomarkers for monitoring disease progression.\n                <\/jats:p>","DOI":"10.1101\/2025.06.09.658729","type":"posted-content","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T11:35:15Z","timestamp":1749641715000},"source":"Crossref","is-referenced-by-count":1,"title":["Maintenance of chronic neuroinflammation in multiple sclerosis via interferon signaling and CD8 T cell-mediated cytotoxicity"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4782-2100","authenticated-orcid":false,"given":"Syed Ali","family":"Raza","sequence":"first","affiliation":[{"name":"Translational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimi","family":"Enose-Akahata","sequence":"additional","affiliation":[{"name":"Viral Immunology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Blazier","sequence":"additional","affiliation":[{"name":"Sanofi, Cambridge, MA, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren M.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Translational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA;"},{"name":"Northwestern University, Chicago, IL, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin S.","family":"Beck","sequence":"additional","affiliation":[{"name":"Translational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA;"},{"name":"Department of Neurology, Icahn school of Medicine at Mount Sinai, New York, NY 10029, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda I.","family":"Ga\u00edtan","sequence":"additional","affiliation":[{"name":"Translational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nyater","family":"Ngouth","sequence":"additional","affiliation":[{"name":"Viral Immunology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jefte M.","family":"Drijvers","sequence":"additional","affiliation":[{"name":"Abata Therapeutics, Watertown, MA, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2947-7606","authenticated-orcid":false,"given":"Eric B.","family":"Dammer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA;"},{"name":"Goizueta Alzheimer\u2019s Disease Research Center, Emory University School of Medicine, Atlanta, GA, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devan","family":"Moodley","sequence":"additional","affiliation":[{"name":"Abata Therapeutics, Watertown, MA, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kory R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Bioinformatics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Absinta","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Humanitas University, Milan, Italy;"},{"name":"Neuro Center, IRCCS Humanitas Research Hospital, Milan, 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