{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:53:12Z","timestamp":1785405192607,"version":"3.56.0"},"posted":{"date-parts":[[2025,6,12]]},"group-title":"Neuroscience","reference-count":21,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["5R01AA027462"],"award-info":[{"award-number":["5R01AA027462"]}],"id":[{"id":"https:\/\/ror.org\/02jzrsm59","id-type":"ROR","asserted-by":"publisher"}]},{"award":["1F31AA031920"],"award-info":[{"award-number":["1F31AA031920"]}],"id":[{"id":"https:\/\/ror.org\/02jzrsm59","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2P50AA022534"],"award-info":[{"award-number":["2P50AA022534"]}],"id":[{"id":"https:\/\/ror.org\/02jzrsm59","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["5P20GM109089"],"award-info":[{"award-number":["5P20GM109089"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,6,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Fetal alcohol spectrum disorders affect approximately 1 in 20 school age children in the United States of America. To study fetal alcohol spectrum disorders, mouse models are commonly used. Of the many approaches of gestational exposure, voluntary drinking paradigms represent the most similar mechanism of drinking as human exposure. These exposures can be done through low-tech solutions such as test tubes (TT), or more high-tech methods such as a volumetric drinking monitor (VDM). Here were compare the TT method and the VDM directly, to evaluate their effect on female mouse drinking.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Method<\/jats:title>\n                  <jats:p>We adapted a drinking in the dark, active cycle, limited access (4 hr.) voluntary drinking paradigm first described by Brady et al. (2012) to test tubes and the volumetric drinking monitor. 8 mice were placed in either drinking method and we evaluated their drinking volume and blood alcohol concentrations (BACs). We compared the values for each group using t-tests.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    After 2 weeks of drinking 10% ethanol with 0.4% saccharine, BACs were not significantly different [t(14)=0.2681, p=0.7935] between the VDM (81.56 \u00b1 21.16 mg\/dL)\n                    <jats:italic>vs<\/jats:italic>\n                    .TT (73.14 \u00b1 23.20 mg\/dL) groups. Calculated intake of ethanol (g\/kg) on the day of blood draw for BAC analysis was also not significantly different [t(14)=0.4308, p=0.6732] between VDM (2.985 \u00b1 0.4127) vs.TT (3.260 \u00b1 0.4863; Fig 1B) groups.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Test tube or VDM resulted in similar average daily ethanol consumption and resultant BACs in female mice<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.06.10.658718","type":"posted-content","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T22:50:13Z","timestamp":1749768613000},"source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of Test Tube and Volumetric Drinking Monitor Methods in Voluntary Ethanol Consumption in Female Mice for Prenatal Alcohol Exposure"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2563-6764","authenticated-orcid":false,"given":"Arasely M.","family":"Rodriguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korbin C.","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Anna","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025061423351208000_2025.06.10.658718v1.1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2020.00359"},{"key":"2025061423351208000_2025.06.10.658718v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(87)90502-8"},{"key":"2025061423351208000_2025.06.10.658718v1.3","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-007-1022-6"},{"key":"2025061423351208000_2025.06.10.658718v1.4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2011.01644.x"},{"key":"2025061423351208000_2025.06.10.658718v1.5","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.21129"},{"key":"2025061423351208000_2025.06.10.658718v1.6","doi-asserted-by":"publisher","DOI":"10.3233\/BPL-200112"},{"key":"2025061423351208000_2025.06.10.658718v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2009.06.004"},{"key":"2025061423351208000_2025.06.10.658718v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(73)91092-1"},{"key":"2025061423351208000_2025.06.10.658718v1.9","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3812-07.2007"},{"key":"2025061423351208000_2025.06.10.658718v1.10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2000.tb04560.x"},{"key":"2025061423351208000_2025.06.10.658718v1.11","unstructured":"Lemoine, P. , Harouseau, H. , Borteryu, J. , & Menuet, J. J. (1968). Les enfants des parents alcooliques. Anomalies observ\u00e9es \u00e0 propos de 127 cas. https:\/\/api.semanticscholar.org\/CorpusID:148717406"},{"key":"2025061423351208000_2025.06.10.658718v1.12","doi-asserted-by":"publisher","DOI":"10.1111\/acer.15210"},{"key":"2025061423351208000_2025.06.10.658718v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"2025061423351208000_2025.06.10.658718v1.14","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14876"},{"key":"2025061423351208000_2025.06.10.658718v1.15","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14040"},{"key":"2025061423351208000_2025.06.10.658718v1.16","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.21896"},{"key":"2025061423351208000_2025.06.10.658718v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2024.110044"},{"key":"2025061423351208000_2025.06.10.658718v1.18","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2014.00093"},{"key":"2025061423351208000_2025.06.10.658718v1.19","doi-asserted-by":"publisher","DOI":"10.3390\/nu13103452"},{"issue":"1","key":"2025061423351208000_2025.06.10.658718v1.20","first-page":"e53","article-title":"Life Expectancy of People with Fetal Alcohol Syndrome","volume":"23","year":"2016","journal-title":"Journal of Population Therapeutics and Clinical Pharmacology = Journal De La Therapeutique Des Populations Et De La Pharmacologie Clinique"},{"key":"2025061423351208000_2025.06.10.658718v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2012.01.006"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.06.10.658718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:11:32Z","timestamp":1782277892000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.06.10.658718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,12]]},"references-count":21,"URL":"https:\/\/doi.org\/10.1101\/2025.06.10.658718","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1016\/j.toxrep.2026.102245","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2025,6,12]]},"subtype":"preprint"}}