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While direct effects of alcohol on brain function are well-established, the role of microglia in acute and chronic neurological dysfunction in AUD remains unclear. Using longitudinal\n                  <jats:italic>in vivo<\/jats:italic>\n                  imaging in mice during acute and repeated alcohol abuse, we found that microglia exhibit dynamic morphological responses that precede but parallel ethanol-induced sedation. Ethanol also induced microglia-dependent synapse elimination and reduced neuronal activity and density. Genetic disruption of microglial MyD88 reversed these ethanol-associated changes in microglial reactivity, neuronal structure, and function, while protecting against alcohol-induced intoxication and motor impairments. These findings identify microglia as cellular drivers of acute and chronic brain dysfunction following alcohol abuse, and highlight MyD88 as a critical therapeutic target for the detrimental neurological consequences of AUD.\n                <\/jats:p>","DOI":"10.1101\/2025.06.12.658437","type":"posted-content","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:40:11Z","timestamp":1750034411000},"source":"Crossref","is-referenced-by-count":1,"title":["Preventing Microglial Reactivity Protects from Acute and Progressive Neuronal Dysfunction, Motor Impairments and Sedation following Alcohol Abuse"],"prefix":"10.64898","author":[{"given":"Evi","family":"Paouri","sequence":"first","affiliation":[{"name":"Department of Neurosciences, Cleveland Clinic Research, Cleveland, OH 44106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Stanko","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, Cleveland Clinic Research, 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